Glycine N-Methyltransferase/GNMT Antibody (OTI8A3) - Azide and BSA Free

Western Blot: Glycine N-Methyltransferase/GNMT Antibody (OTI8A3) - Azide and BSA Free [NBP2-71838] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GNMT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GNMT.

CyTOF-ready: Glycine N-Methyltransferase/GNMT Antibody (OTI8A3) - Azide and BSA Free [NBP2-71838] - HEK293T cells transfected with either pCMV6-ENTRY GNMT.(Red) or empty vector control plasmid (Blue) were immunostaining with anti-GNMT mouse monoclonal, and then analyzed by flow cytometry.

• Reactivity: Hu, Mu
• Applications: WB, Flow, CyTOF-ready
• Clone: OTI8A3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Glycine N-Methyltransferase/GNMT Antibody (OTI8A3) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Glycine N-Methyltransferase/GNMT Antibody (OTI8A3) - Azide and BSA Free (NBP2-02634) is a monoclonal antibody validated for use in WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human GNMT (NP_061833) produced in HEK293T cell.
• Isotype: IgG3
• Clonality: Monoclonal
• Host: Mouse
• Gene: GNMT
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Western Blot 1:200 - 1:1000
• Theoretical MW: 32.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Glycine N-Methyltransferase/GNMT Antibody (OTI8A3) - Azide and BSA Free

• EC 2.1.1.20
• Glycine NMethyltransferase
• Glycine N-Methyltransferase
• GNMT

Background

GNMT is encoded by this gene is an enzyme that catalyzes the conversion of S-adenosyl-L-methionine (along with glycine) to S-adenosyl-L-homocysteine and sarcosine. The encoded protein is found in the cytoplasm and acts as a homotetramer. Defects in this gene are a cause of GNMT deficiency (hypermethioninemia).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GNMT