Glutathione S-Transferase pi 1/GSTP1 Antibody

Product Discontinued

Glutathione S-Transferase pi 1/GSTP1 Antibody Summary

• Immunogen: A peptide mapping at the C-terminus of GSTpi of human origin, different from the related mouse sequence by two sequence.
• Epitope: For IHC-P: Boiling the sections is required. For ICC: Antigen retrieval by Pepsin and Trypsin is required.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GSTP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.5-1 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Western Blot 1-2 ug/ml
• Application Notes: This antibody is useful in Immunocytochemistry, Immunohistochemistry-Paraffin, Western Blot.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 mg DiSodium Phosphate, 0.9 mg NaCl and 5 mg BSA
• Preservative: 0.05mg Thimerosal
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute with deionized water.

Alternate Names for Glutathione S-Transferase pi 1/GSTP1 Antibody

• deafness, X-linked 7
• DFN7
• EC 2.5.1.18
• FAEES3
• fatty acid ethyl ester synthase III
• glutathione S-transferase P
• Glutathione STransferase pi 1
• Glutathione S-Transferase pi 1
• GST class-pi
• GST3DFN7
• GSTP
• GSTP1
• GSTP1-1
• PI

Background

GSTP1 (glutathione-S-transferase, pi 1), also called GST3/DFN7, which is located on chromosome 11q13 , is a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. GSTP1 act like a tumor suppressor gene, which when inactivated leads to tumor growth, and the -class glutathione S-transferase is commonly inactivated by somatic CpGisland hypermethylation in cancers of the prostate, liver, and breast. Methylation of regulatory sequences at the GSTP1 gene locus is found in the vast majority (>90%) of prostate carcinomas and is associated with transcriptional down-regulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Glutathione S-Transferase pi 1/GSTP1 Antibody (NBP1-42011)(1)

Publication using NBP1-42011

• Lin LC, Yeh CT, Kuo CC et al. Sulforaphane Potentiates the Efficacy of Imatinib against Chronic Leukemia Cancer Stem Cells through Enhanced Abrogation of Wnt/beta-Catenin Function J Agric Food Chem 2012-07-18 [PMID: 22708678] (WB, Human)

FAQs for Glutathione S-Transferase pi 1/GSTP1 Antibody (NBP1-42011). (Showing 1 - 1 of 1 FAQ).

1. I received NBP1-42011 today; I thought that this antibody is liquid type, but it is powder type. Do I have to reconstitute it before use? If I have to reconstitute it, please let me know the reconstitution buffer.
   • NBP1-42011 is lyophilized and it needs to be reconstituted before use. You should spin down the vial and add 0.1-0.2 mL of distilled or purified water, vortex and spin again to give you a stock concentration of 0.5-1.0 mg/mL.

Bioinformatics

• Gene Symbol: GSTP1
• Entrez:
  • 2950(Human)
  • 14870(Mouse)
  • 24426(Rat)
• Uniprot:
  • P09211(Human)