Glutathione S-Transferase pi 1/GSTP1 Antibody

Western Blot: Glutathione S-Transferase pi 1/GSTP1 Antibody [NB100-1792] - (0.01ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: Glutathione S-Transferase pi 1/GSTP1 Antibody [NB100-1792] - (5ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Western Blot: Glutathione S-Transferase pi 1/GSTP1 Antibody [NB100-1792] - Staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western Blot: Glutathione S-Transferase pi 1/GSTP1 Antibody [NB100-1792] - HEK293 overexpressing GSTP1 and probed with (mock transfection in first lane).

Western Blot: Glutathione S-Transferase pi 1/GSTP1 Antibody [NB100-1792] - (RC203086) and probed with EB06802 (mock transfection in first lane), tested by Origene.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Glutathione S-Transferase pi 1/GSTP1 Antibody Summary

• Description: Novus Biologicals Goat Glutathione S-Transferase pi 1/GSTP1 Antibody (NB100-1792) is a polyclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-LADQGQSWKEEV corresponding to internal region according to NP_000843.1.
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GSTP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1:1000
• Application Notes: WB: Approx. 24 kDa band observed in human kidney, liver and lung lysates (calculated MW of 23.4 kDa band according to NP_000843.1). In transfected HEK293 transiently expressing GSTP1 band of approx. 26 kDa band is observed. This band is not observed in the non-transfected HEK293.
• Control:

  Human Liver Whole Tissue Lysate (Adult Whole Normal)
  Glutathione S-Transferase pi 1/GSTP1 Overexpression Lysate
  Human Kidney Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Glutathione S-Transferase pi 1/GSTP1 Antibody

• deafness, X-linked 7
• DFN7
• EC 2.5.1.18
• FAEES3
• fatty acid ethyl ester synthase III
• glutathione S-transferase P
• Glutathione STransferase pi 1
• Glutathione S-Transferase pi 1
• GST class-pi
• GST3DFN7
• GSTP
• GSTP1
• GSTP1-1
• PI

Background

Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. The glutathione S-transferase pi gene (GSTP1) is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GSTP1
• Entrez:
  • 2950(Human)
• Uniprot:
  • P09211(Human)