Glutathione S-Transferase pi 1/GSTP1 Antibody (3F2C2)

Western Blot: Glutathione S-Transferase pi 1/GSTP1 Antibody (3F2C2) [NB110-60512] - Analysis using GSTP1 mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY GSTP1 cDNA (2).

Immunocytochemistry/Immunofluorescence: Glutathione S-Transferase pi 1/GSTP1 Antibody (3F2C2) [NB110-60512] - Analysis of HepG2 (left) and L-02 (right) cells using GSTP1 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.

Immunohistochemistry-Paraffin: Glutathione S-Transferase pi 1/GSTP1 Antibody (3F2C2) [NB110-60512] - Analysis of human prostate tissues using GSTP1 mouse mAb with DAB staining.

Flow Cytometry: Glutathione S-Transferase pi 1/GSTP1 Antibody (3F2C2) [NB110-60512] - Analysis of K562 cells using GSTP1 mouse mAb (green) and negative control (purple).

Western Blot: Glutathione S-Transferase pi 1/GSTP1 Antibody (3F2C2) [NB110-60512] - Analysis using GSTP1 mouse mAb against PC3 cell lysate (1) and human cerebellum tissue lysate (2).

Immunocytochemistry/Immunofluorescence: Glutathione S-Transferase pi 1/GSTP1 Antibody (3F2C2) [NB110-60512] - Analysis of PC-3 cells using GSTP1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clone: 3F2C2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Glutathione S-Transferase pi 1/GSTP1 Antibody (3F2C2) Summary

• Immunogen: Purified recombinant fragment of human Glutathione S-Transferase pi 1/GSTP1 expressed in E. Coli.
• Specificity: GSTP1 (3F2C2)
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GSTP1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 23 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Glutathione S-Transferase pi 1/GSTP1 Overexpression Lysate

Reactivity Notes

Mouse reactivity reported in the scientific literature (PMID: 23703832). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for Glutathione S-Transferase pi 1/GSTP1 Antibody (3F2C2)

• deafness, X-linked 7
• DFN7
• EC 2.5.1.18
• FAEES3
• fatty acid ethyl ester synthase III
• glutathione S-transferase P
• Glutathione STransferase pi 1
• Glutathione S-Transferase pi 1
• GST class-pi
• GST3DFN7
• GSTP
• GSTP1
• GSTP1-1
• PI

Background

GSTP1 (glutathione-S-transferase, pi 1), also called GST3/DFN7, which is located on chromosome 11q13 , is a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. GSTP1 act like a tumor suppressor gene, which when inactivated leads to tumor growth, and the -class glutathione S-transferase is commonly inactivated by somatic CpGisland hypermethylation in cancers of the prostate, liver, and breast. Methylation of regulatory sequences at the GSTP1 gene locus is found in the vast majority (>90%) of prostate carcinomas and is associated with transcriptional down-regulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Glutathione S-Transferase pi 1/GSTP1 Antibody (NB110-60512)(2)

Publications using NB110-60512

• Kaisar MA, Sivandzade F, Bhalerao A, Cucullo L Conventional and electronic cigarettes dysregulate the expression of iron transporters and detoxifying enzymes at the brain vascular endothelium: In vivo evidence of a gender-specific cellular response to chronic cigarette smoke exposure Neurosci Lett. 2018-08-23 [PMID: 29879439] (WB, Mouse)
• Hasenfuss SC, Bakiri L, Thomsen MK et al. The AP-1 transcription factor Fra-1 is dispensable for murine liver fibrosis, but modulates xenobiotic metabolism. Hepatology 2013-05-23 [PMID: 23703832] (WB, Mouse)

Bioinformatics

• Gene Symbol: GSTP1
• Entrez:
  • 2950(Human)
• Uniprot:
  • P09211(Human)