Recombinant E. coli Glutathione Reductase Protein

Images

 
SDS-Page: Glutathione Reductase Protein [NBP2-22639]

Product Details

Summary
Reactivity EcSpecies Glossary
Applications Func, PAGE, Bioactivity
Concentration
1 mg/ml

Order Details

Recombinant E. coli Glutathione Reductase Protein Summary

Description
A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-450 of E.coli Glutathione Reductase

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMTKHYDY IAIGGGSGGI ASINRAAMYG QKCALIEAKE LGGTCVNVGC VPKKVMWHAA QIREAIHMYG PDYGFDTTIN KFNWETLIAS RTAYIDRIHT SYENVLGKNN VDVIKGFARF VDAKTLEVNG ETITADHILI ATGGRPSHPD IPGVEYGIDS DGFFALPALP ERVAVVGAGY IAVELAGVIN GLGAKTHLFV RKHAPLRSFD PMISETLVEV MNAEGPQLHT NAIPKAVVKN TDGSLTLELE DGRSETVDCL IWAIGREPAN DNINLEAAGV KTNEKGYIVV DKYQNTNIEG IYAVGDNTGA VELTPVAVAA GRRLSERLFN NKPDEHLDYS NIPTVVFSHP PIGTVGLTEP QAREQYGDDQ VKVYKSSFTA MYTAVTTHRQ PCRMKLVCVG SEEKIVGIHG IGFGMDEMLQ GFAVALKMGA TKKDFDNTVA IHPTAAEEFV TMR

Details of Functionality
Specific activity is > 60 units/mg. The unit definition for glutathione reductase activity may be expressed in terms of the oxidation of NADPH or the reduction of GSSG since their molar ratio is 1:1. One unit of glutathione reductase oxidizes 1 umol of NADPH per minute at 37C, pH 7.5.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
GSR
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
51.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 0.1M NaCl, 1mM DTT
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant E. coli Glutathione Reductase Protein

  • EC 1.8.1
  • EC 1.8.1.7
  • GLUR
  • Glutathione Reductase
  • glutathione reductase, mitochondrial
  • GR
  • GRase
  • GRD1
  • GSR
  • HEL-752
  • MGC78522

Background

gor, also known as Glutathione reductase, belongs to the class-I pyridine nucleotide disulfide oxidoreductase family. The main function of the protein is to maintain high levels of reduced glutathione in the cytosol. With the concomitant oxidation of NADPH, Glutathione reductase transforms oxidized glutathione to the reduced form. The active site of the protein is a redox-active disulfide bond. Recombinant E. coli gor protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-24916
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC, IHC-P
NB300-731
Species: Hu, Mu, Rt, GP, Rb, Sh, Ye
Applications: WB, ChIP, Flow, GS, ICC/IF, IHC, IHC-P, IP, B/N
NBP2-24915
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC-P
NBP1-86614
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-55298
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-89727
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-49118
Species: Hu
Applications: IHC, IHC-P
NB100-236
Species: Hu, Mu
Applications: WB, IB, IHC-Fr, IP
NB100-1533
Species: Hu, Mu, Po, Sh
Applications: WB, ICC/IF, IHC, IHC-P, PEP-ELISA
NB300-562
Species: Hu, Mu, Rt, Ch, Rb, Sh
Applications: WB, Flow, ICC/IF, IHC, IHC-P, B/N
NB110-61574
Species: Hu, Mu, Bv, Gt, Sh
Applications: WB
NB100-116
Species: Hu, Mu, Rt, Pm
Applications: WB, Simple Western, ChIP, GS, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA
AF5415
Species: Hu
Applications: WB, IHC, ICC
NBP2-67471
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB100-56603
Species: Hu, Pm
Applications: WB, IHC, IHC-P
NBP2-22639
Species: Ec
Applications: Func, PAGE, Bioactivity

Publications for Glutathione Reductase Protein (NBP2-22639) (0)

There are no publications for Glutathione Reductase Protein (NBP2-22639).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Glutathione Reductase Protein (NBP2-22639) (0)

There are no reviews for Glutathione Reductase Protein (NBP2-22639). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

FAQs for Glutathione Reductase Protein (NBP2-22639) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Array Products

Bioinformatics Tool for Glutathione Reductase Protein (NBP2-22639)

Discover related pathways, diseases and genes to Glutathione Reductase Protein (NBP2-22639). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Glutathione Reductase Protein (NBP2-22639)

Discover more about diseases related to Glutathione Reductase Protein (NBP2-22639).
 

Pathways for Glutathione Reductase Protein (NBP2-22639)

View related products by pathway.

PTMs for Glutathione Reductase Protein (NBP2-22639)

Learn more about PTMs related to Glutathione Reductase Protein (NBP2-22639).
 

Research Areas for Glutathione Reductase Protein (NBP2-22639)

Find related products by research area.

Blogs on Glutathione Reductase

There are no specific blogs for Glutathione Reductase, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant E. coli Glutathione Reductase Protein and receive a gift card or discount.

Bioinformatics

Gene Symbol GSR
Entrez