Glutathione Peroxidase 5 Antibody - BSA Free

Western Blot: Glutathione Peroxidase 5 Antibody - BSA Free [NBP3-46178] - Human testis tissue were subjected to SDS PAGE followed by western blot with (Glutathione Peroxidase 5 antibody) at dilution of 1:200

Immunohistochemistry: Glutathione Peroxidase 5 Antibody - BSA Free [NBP3-46178] - Immunohistochemistry of paraffin-embedded human testis using (Glutathione Peroxidase 5 antibody) at dilution of 1:50

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Glutathione Peroxidase 5 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Glutathione Peroxidase 5 Antibody - BSA Free (NBP3-46178) is a polyclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: glutathione peroxidase 5(epididymal androgen-related protein). (Uniprot# O75715)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GPX5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1:20-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 25 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% Glycerol, pH (7.3)
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Glutathione Peroxidase 5 Antibody - BSA Free

• EC 1.11.1
• EC 1.11.1.9
• EGLP
• epididymal androgen-related protein
• epididymal secretory glutathione peroxidase
• Epididymis-specific glutathione peroxidase-like protein
• glutathione peroxidase 5 (epididymal androgen-related protein)
• Glutathione peroxidase 5
• GPx-5
• GSHPx-5

Background

Glutathione peroxidase (GPX)/glutathione reductase is part of the hydrogen peroxide scavenging system found within the epididymis in the mammalian male reproductive tract. GPX5 expression is epididymis-specific and the transcript is unique from other GPXs because it contains a deletion resulting in an mRNA that does not contain a selenocysteine (UGA) codon (an unusual amino acid present in other GPXs). This deletion also renders the mRNA incapable of encoding an active GPX isoenzyme. For this reason, GPX5 is selenium-independent and has very little activity towards hydrogen peroxide or organic hydroperoxides. GPX5, which is bound to the acrosome of sperm, may act to protect sperm from premature acrosome reaction in the epididymis. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GPX5