Glutathione Peroxidase 4/GPX4 Antibody (565320) [DyLight 650]

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 565320
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: DyLight 650

Glutathione Peroxidase 4/GPX4 Antibody (565320) [DyLight 650] Summary

• Immunogen: E. coli-derived recombinant human Glutathione Peroxidase 4
  Gly74-Phe168
  Accession # P36969
• Specificity: Detects endogenous human, mouse and rat GPX4 in Western blots.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: GPX4
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for Glutathione Peroxidase 4/GPX4 Antibody (565320) [DyLight 650]

• EC 1.11.1
• EC 1.11.1.12
• glutathione peroxidase 4 (phospholipid hydroperoxidase)
• Glutathione Peroxidase 4
• GPX4
• GPx-4
• GSHPx-4
• MCSP
• PHGPx
• PHGPxsnGPx
• phospholipid hydroperoxidase
• phospholipid hydroperoxide glutathione peroxidase, mitochondrial
• snGPx
• snPHGPx
• sperm nucleus glutathione peroxidase

Background

Glutathione peroxidase catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. Human plasma glutathione peroxidase has been shown to be a selenium-containing enzyme and the UGA codon is translated into a selenocysteine. Through alternative splicing and transcription initiation, rat produces proteins that localize to the nucleus, mitochondrion, and cytoplasm. In humans, experimental evidence for alternative splicing exists; alternative transcription initiation and the cleavage sites of the mitochondrial and nuclear transit peptides need to be experimentally verified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GPX4