Glutathione Peroxidase 3/GPX3 Antibody [Unconjugated]

Western blot shows lysates of human plasma, mouse kidney tissue, and rat kidney tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat Glutathione Peroxidase 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4199) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Glutathione Peroxidase 3 at approximately 24kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Glutathione Peroxidase 3/GPX3 was detected in immersion fixed paraffin-embedded sections of human kidney using 15 µg/mL Goat Anti-Human/Mouse/Rat Glutathione Peroxidase 3/GPX3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4199) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm in tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western lane view shows human serum, loaded at 0.2 mg/mL. A specific band was detected for Glutathione Peroxidase 3/GPX3 at approximately 29 kDa (as indicated) using 25 µg/mL of Goat Anti-Human/Mouse/Rat Glutathione Peroxidase 3/GPX3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4199) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

Bioinformatics analysis showed that GPX3 expression was significantly increased during adipogenic differentiation of hBMSCs. A Heatmap of adipogenic marker genes and GPx family genes. B Volcano plot of adipogenic marker genes and GPx family genes. C The gene expression pattern of GPx family and oxidative stress-related enzymes during osteogenic and adipogenic differentiation of hBMSCs. Oxidative stress-related enzymes genes: PLA2G2A, LOX, CAT; Adipogenic marker genes: PPARG, CEBPA, FABP4; osteogenic marker genes: ALPL, BGLAP Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/40410842), licensed under a CC-BY license. Not internally tested by R&D Systems.

GPx3 expression during adipogenic differentiation of mBMSC in vitro. A Representative images of Oil Red O staining at different differentiation time points and (B) corresponding quantification. C Representative Western blot images showing the protein expressions of GPx3, along with adipogenic markers PPAR gamma and C/EBP alpha , at various differentiation stages. D-F Quantifications of band intensities in (C). G-J Relative mRNA expression levels of Gpx3 and adipogenic-specific genes Pparg, Cebp alpha , and Adipoq normalized to Actb during adipogenic differentiation. Data are presented as mean ± SD. *P 0.05 Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/40410842), licensed under a CC-BY license. Not internally tested by R&D Systems.

scRNA-seq data indicates that increased BMAds is associated with diminished Gpx3 expression during ageing. A The proportion of bone marrow cells in the young and aged bone marrow. B The expression levels of Adipoq or Lpl in the young and aged bone marrow. C-E UMAP visualization of Adipoq (adipocyte marker), Aspn (BMSC marker) and Gpx3 expression in scRNA-seq data from bone marrow cells of mice at different ages. F The expression levels of Gpx3 in BMSCs and adipocytes in the young and aged mice. 3 M, three-month-old mice; 16 M, sixteen-month-old mice; EC, endothelial cell Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/40410842), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Glutathione Peroxidase 3/GPX3 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse Glutathione Peroxidase 3/GPX3
  Gly74-Lys226
  Accession # P46412
• Specificity: Detects human, mouse, and rat Glutathione Peroxidase 3/GPX3 in Western blots and detects recombinant human and recombinant mouse Glutathione Peroxidase 3/GPX3 in direct ELISAs. In direct ELISAs, approximately 15% cross-reactivity with recombinant human (rh) GPX5 and recombinant mouse (rm) GPX1 is observed, and less than 4% cross-reactivity with rhGPX4, rhGPX6, and rmGPX2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GPX3
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 25 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Glutathione Peroxidase 3/GPX3 Antibody [Unconjugated]

• EC 1.11.1
• EC 1.11.1.9
• Extracellular glutathione peroxidase
• glutathione peroxidase 3 (plasma)
• Glutathione Peroxidase 3
• GPX3
• GPx-3
• GPXP
• GPX-P
• GSHPx-3
• GSHPx-P
• Plasma glutathione peroxidase

Background

Glutathione Peroxidase 3 (GPX3), also known as Plasma Glutathione Peroxidase, is a 226 amino acid member of the glutathione peroxidase antioxidant enzyme family. The Glutathione Peroxidase family protects cell surfaces, extracellular fluid components, and enzymes from oxidative stress by catalysing the reduction of hydrogen peroxide, lipid peroxides, and organic hydroperoxide using reduced glutathione. GPX3 is secreted into plasma and like GPX1, is an ~100 kDa homotetramer consisting of four identical 23 kDa subunits, each containing a selenocysteine residue at the active site. GPX3 exhibits highest levels of mRNA expression in liver (human only), kidney, heart and lung tissues. Mouse GPX3 shares 89% and 99% amino acid identity with human and rat GPX3, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Glutathione Peroxidase 3/GPX3 Antibody (AF4199)(15)

Publications using AF4199

• Kuehl, M;Okabayashi, Y;Wong, MN;Gernhold, L;Gut, G;Kaiser, N;Schwerk, M;Gräfe, SK;Ma, FY;Tanevski, J;Schäfer, PSL;Mezher, S;Sarabia Del Castillo, J;Goldbeck-Strieder, T;Zolotareva, O;Hartung, M;Delgado Chaves, FM;Klinkert, L;Gnirck, AC;Spehr, M;Fleck, D;Joodaki, M;Parra, V;Shaigan, M;Diebold, M;Prinz, M;Kranz, J;Kux, JM;Braun, F;Kretz, O;Wu, H;Grahammer, F;Heins, S;Zimmermann, M;Haas, F;Kylies, D;Wanner, N;Czogalla, J;Dumoulin, B;Zolotarev, N;Lindenmeyer, M;Karlson, P;Nyengaard, JR;Sebode, M;Weidemann, S;Wiech, T;Groene, HJ;Tomas, NM;Meyer-Schwesinger, C;Kuppe, C;Kramann, R;Karras, A;Bruneval, P;Tharaux, PL;Pastene, D;Yard, B;Schaub, JA;McCown, PJ;Pyle, L;Choi, YJ;Yokoo, T;Baumbach, J;Sáez, PJ;Costa, I;Turner, JE;Hodgin, JB;Saez-Rodriguez, J;Huber, TB;Bjornstad, P;Kretzler, M;Lenoir, O;Nikolic-Paterson, DJ;Pelkmans, L;Bonn, S;Puelles, VG; Pathology-oriented multiplexing enables integrative disease mapping Nature 2025-07-18 [PMID: 40681898] (IF/IHC, Human)
• Chang, C;Cheng, YY;Kamlapurkar, S;White, SR;Tang, PW;Elhaw, AT;Javed, Z;Aird, KM;Mythreye, K;Phaëton, R;Hempel, N; GPX3 supports ovarian cancer tumor progression in vivo and promotes expression of GDF15 bioRxiv : the preprint server for biology 2024-01-29 [PMID: 38352432] (Western Blot, Human)
• Lei, FJ;Chiang, JY;Chang, HJ;Chen, DC;Wang, HL;Yang, HA;Wei, KY;Huang, YC;Wang, CC;Wei, ST;Hsieh, CH; Cellular and exosomal GPx1 are essential for controlling hydrogen peroxide balance and alleviating oxidative stress in hypoxic glioblastoma Redox biology 2023-09-01 [PMID: 37572455] (Western Blot, Human)
• Dabrowska M, K?pczy?ska A, Go?dzik K et al. Novel Role of Mammalian Cell Senescence-Sustenance of Muscle Larvae of Trichinella spp Oxidative Medicine and Cellular Longevity 11/28/2022 [PMID: 36478989]
• Dabrowska M, K?pczy?ska A, Go?dzik K et al. Novel Role of Mammalian Cell Senescence-Sustenance of Muscle Larvae of Trichinella spp Oxidative Medicine and Cellular Longevity 2022-11-28 [PMID: 36478989]
• Timothy Wai Ho Shuen, Marianna Alunni-Fabbroni, Elif Öcal, Peter Malfertheiner, Moritz Wildgruber, Regina Schinner et al. Extracellular Vesicles May Predict Response to Radioembolization and Sorafenib Treatment in Advanced Hepatocellular Carcinoma: An Exploratory Analysis from the SORAMIC Trial Clinical Cancer Research 2022-09-01 [PMID: 35763041] (Immunohistochemistry, Human)
• Bo Shan, Clive S. Barker, Mengle Shao, Qianbin Zhang, Rana K. Gupta, Yibo Wu Multilayered omics reveal sex- and depot-dependent adipose progenitor cell heterogeneity Cell Metabolism 2022-05-01 [PMID: 35447091]
• Sara Evke, Qishan Lin, Juan Andres Melendez, Thomas John Begley Epitranscriptomic Reprogramming Is Required to Prevent Stress and Damage from Acetaminophen Genes (Basel) 2022-02-25 [PMID: 35327975]
• MT Ringuet, B Hunne, M Lenz, DM Bravo, JB Furness Analysis of Bioavailability and Induction of Glutathione Peroxidase by Dietary Nanoelemental, Organic and Inorganic Selenium Nutrients, 2021-03-25;13(4):. 2021-03-25 [PMID: 33806211] (Western Blot, Mouse)
• Andrea Leonardi, Nataliia Kovalchuk, Lei Yin, Lauren Endres, Sara Evke, Steven Nevins et al. The epitranscriptomic writer ALKBH8 drives tolerance and protects mouse lungs from the environmental pollutant naphthalene Epigenetics 2020-10-02 [PMID: 32303148] (Simple Western, Mouse, Transgenic Mouse)
• Seong Beom Ahn, Samridhi Sharma, Abidali Mohamedali, Sadia Mahboob, William J. Redmond, Dana Pascovici et al. Potential early clinical stage colorectal cancer diagnosis using a proteomics blood test panel Clinical Proteomics 2019-12-01 [PMID: 31467500]
• He M, Wang J, Yin Z et al. MiR-320a induces diabetic nephropathy via inhibiting MafB Aging (Albany NY) 5/17/2019 [PMID: 31102503]
• Van Brussel , Ilse, Schrijvers , Dorien M, Martinet , Wim, Pintelon , Isabel, Deschacht , Maartje, Schnorbusch , Kathy, Maes , Louis, Bosmans , Johan M, Vrints , Christia, Adriaensen , Dirk, Cos , Paul, Bult , Hidde Transcript and protein analysis reveals better survival skills of monocyte-derived dendritic cells compared to monocytes during oxidative stress. PLoS ONE, 2012-08-15;7(8):e43357. 2012-08-15 [PMID: 22916248] (ICC, Western Blot, Human)
• Seale L, Hashimoto A, Kurokawa S, Gilman C, Seyedali A, Bellinger F, Raman A, Berry M Disruption of the selenocysteine lyase-mediated selenium recycling pathway leads to metabolic syndrome in mice. Mol Cell Biol, 2012-08-13;32(20):4141-54. 2012-08-13 [PMID: 22890841] (Western Blot, Mouse)
• Herault O, Hope KJ, Deneault E, Mayotte N, Chagraoui J, Wilhelm BT, Cellot S, Sauvageau M, Andrade-Navarro MA, Hebert J, Sauvageau G A role for GPx3 in activity of normal and leukemia stem cells. J. Exp. Med., 2012-04-16;209(5):895-901. 2012-04-16 [PMID: 22508837] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: GPX3
• Uniprot:
  • P46412()