Glutathione Peroxidase 3/GPX3 Antibody [Biotin]

Western blot shows human serum. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Glutathione Peroxidase 3/GPX3 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF4199) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Glutathione Peroxidase 3/GPX3 at approximately 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

Glutathione Peroxidase 3/GPX3 Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant mouse Glutathione Peroxidase 3/GPX3
  Gly74-Lys226
  Accession # P46412
• Specificity: Detects human, mouse, and rat Glutathione Peroxidase 3/GPX3 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GPX3
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Glutathione Peroxidase 3/GPX3 Antibody [Biotin]

• EC 1.11.1
• EC 1.11.1.9
• Extracellular glutathione peroxidase
• glutathione peroxidase 3 (plasma)
• Glutathione Peroxidase 3
• GPX3
• GPx-3
• GPXP
• GPX-P
• GSHPx-3
• GSHPx-P
• Plasma glutathione peroxidase

Background

Glutathione Peroxidase 3 (GPX3), also known as Plasma Glutathione Peroxidase, is a 226 amino acid member of the glutathione peroxidase antioxidant enzyme family. The Glutathione Peroxidase family protects cell surfaces, extracellular fluid components, and enzymes from oxidative stress by catalysing the reduction of hydrogen peroxide, lipid peroxides, and organic hydroperoxide using reduced glutathione. GPX3 is secreted into plasma and like GPX1, is an ~100 kDa homotetramer consisting of four identical 23 kDa subunits, each containing a selenocysteine residue at the active site. GPX3 exhibits highest levels of mRNA expression in liver (human only), kidney, heart and lung tissues. Mouse GPX3 shares 89% and 99% amino acid identity with human and rat GPX3, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GPX3
• Uniprot:
  • P46412()