Glutathione Peroxidase 1/GPX1 Antibody (ARC53251)

Western Blot: Glutathione Peroxidase 1/GPX1 Antibody (ARC53251) [NBP3-25943] - Analysis of various lysates, using Glutathione Peroxidase 1/GPX1 antibody at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Western Blot: Glutathione Peroxidase 1/GPX1 Antibody (ARC53251) [NBP3-25943] - Analysis of Rat liver, using Glutathione Peroxidase 1/GPX1 antibody at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunohistochemistry: Glutathione Peroxidase 1/GPX1 Antibody (ARC53251) [NBP3-25943] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using Glutathione Peroxidase 1/GPX1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: Glutathione Peroxidase 1/GPX1 Antibody [Glutathione Peroxidase 1/GPX1] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using Glutathione Peroxidase 1/GPX1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IHC
• Clone: ARC53251
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Glutathione Peroxidase 1/GPX1 Antibody (ARC53251) Summary

• Description: Novus Biologicals Rabbit Glutathione Peroxidase 1/GPX1 Antibody (ARC53251) (NBP3-25943) is a monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein of human GPX1.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: GPX1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA Recommended starting concentration is 1 μg/mL
  • Immunohistochemistry 1:50 - 200
  • Immunohistochemistry-Paraffin 1:50 - 200
  • Western Blot 1:2000 - 1:12000
• Theoretical MW: 22 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.05% Proclin 300
• Purity: Affinity purified

Alternate Names for Glutathione Peroxidase 1/GPX1 Antibody (ARC53251)

• Cellular glutathione peroxidase
• CGPx
• EC 1.11.1
• EC 1.11.1.9
• Glutathione Peroxidase 1
• GPX1
• GPx-1
• GSHPX1
• GSHPx-1
• MGC14399
• MGC88245

Background

FUNCTION: Protects the hemoglobin in erythrocytes from oxidative breakdown. CATALYTIC ACTIVITY: 2 glutathione + H2O2 = glutathione disulfide + 2 H2O. SUBUNIT: Homotetramer. SUBCELLULAR LOCATION: Cytoplasm. SIMILARITY: Belongs to the glutathione peroxidase family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GPX1