Home » Glutaredoxin 1/GLRX1 » Glutaredoxin 1/GLRX1 Proteins » Recombinant Human Glutaredoxin 1/GLRX1 Protein

Recombinant Human Glutaredoxin 1/GLRX1 Protein

 Submit a Review

Images

 
(
Enlarge)
SDS-Page: Recombinant Human Glutaredoxin 1/GLRX1 Protein [NBC1-22929]
SDS-Page: Recombinant Human Glutaredoxin 1/GLRX1 Protein [NBC1-22929]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

Order Details

100% Guarantee on every product

Recombinant Human Glutaredoxin 1/GLRX1 Protein Summary

Description
An un-tagged recombinant protein corresponding to the amino acids1-106 of Human Glutaredoxin 1/GLRX1

Source: E.coli

Amino Acid Sequence: MAQEFVNCKI QPGKVVVFIK PTCPYCRRAQ EILSQLPIKQ GLLEFVDITA TNHTNEIQDY LQQLTGARTV PRVFIGKDCI GGCSDLVSLQ QSGELLTRLK QIGALQ
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
GLRX
Purity
>95%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
11.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 10% glycerol
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>95%, by SDS-PAGE

Alternate Names for Recombinant Human Glutaredoxin 1/GLRX1 Protein

  • GLRX
  • glutaredoxin (thioltransferase)
  • Glutaredoxin 1
  • glutaredoxin-1
  • GRXGRX1
  • MGC117407
  • Thioltransferase
  • Thioltransferase-1
  • TTase-1

Background

Description: Glutaredoxin(GRX), also known as thioltransferase, is member of the thiol-disulfide oxidoreductase family. Glutraredoxin catalyzes the reversible reduction of protein-glutathionyl mixed disulfides to free sulfhydryl groups though a monothiol mechanism. Mammalian Glutaredoxin is known to have two isoforms, GRX1 and GRX2. GRX1 is a cytosolic protein, whereas GRX2 is localized both in the mitochondria and nucleus. Glutaredoxin-1 may be involved in a various cellular events such as signal transduction, stress response, and metabolic regulation by regulating the redox status of cellular proteins. Recombinant human Glutaredoxin1 protein was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

Thioredoxin-1
Antibody
NBP2-52983
Immunocytochemistry/Immunofluorescence: Thioredoxin Antibody [NBP2-52983] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Thioredoxin at a 1:100 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunocytochemistry/Immunofluorescence: Thioredoxin Antibody [NBP2-52983] - NIH-3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Thioredoxin at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunocytochemistry/Immunofluorescence: Thioredoxin Antibody [NBP2-52983] - PC12 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Thioredoxin at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
Glutaredoxin 2
Antibody
NBP1-90040
Immunocytochemistry/Immunofluorescence: Glutaredoxin 2 Antibody [NBP1-90040] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: Glutaredoxin 2 Antibody [NBP1-90040] - Staining of human gall bladder shows strong nuclear positivity in glandular cells.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
VAC14 Antibody
H00055697-B01P
Western Blot: VAC14 Antibody [H00055697-B01P] - Analysis of VAC14 expression in human liver.
Immunocytochemistry/Immunofluorescence: VAC14 Antibody [H00055697-B01P] - Analysis of purified antibody to VAC14 on HeLa cell. (antibody concentration 10 ug/ml)
Western Blot: VAC14 Antibody [H00055697-B01P] - Analysis of VAC14 expression in transfected 293T cell line by VAC14 polyclonal antibody. Lane 1: VAC14 transfected lysate(86.02 KDa). Lane 2: Non-transfected lysate.
Species: Hu
Applications: WB, ELISA, ICC/IF
Glutathione
Reductase Antibody
NBP2-16684
Western Blot: Glutathione Reductase Antibody [NBP2-16684] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutathione Reductase antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: Glutathione Reductase Antibody [NBP2-16684] - Paraffin-embedded rat brain. Glutathione reductase antibody [N2C2], Internal diluted at 1:500.
Western Blot: Glutathione Reductase Antibody [NBP2-16684] - Sample (30 ug of whole cell lysate) A: HCT116 7. 5% SDS PAGE gel, diluted at 1:1000.
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
Thioredoxin
Reductase 1/TRXR1 Ant
NBP2-20619
Western Blot: thioredoxin reductase 1 Antibody [NBP2-20619] - 50 ug Rat brain lysate/extract 7. 5% SDS-PAGE TrxR1 antibody [N3C2], Internal dilution: 1:1000.
Immunocytochemistry/Immunofluorescence: thioredoxin reductase 1 Antibody [NBP2-20619] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: Thioredoxin Reductase 1/TRXR1 Antibody [NBP2-20619] - TrxR1 antibody [N3C2], Internal detects TrxR1 protein at cytoplasm and nucleus in mouse liver by Immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. TrxR1 antibody [N3C2], Internal diluted at 1:500.
(1 Publication)
Species: Hu, Mu, Rt, Pm
Applications: WB, ICC/IF, IHC, IHC-P
Peroxiredoxin 5
Antibody [Unconjugate
AF5724
Western blot shows lysates of human, mouse, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Peroxiredoxin 5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5724) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Peroxiredoxin 5 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Peroxiredoxin 5 was detected in immersion fixed A549 human lung carcinoma cell line using Goat Anti-Human/Mouse/Rat Peroxiredoxin 5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5724) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to mitochondria. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Peroxiredoxin 5 was detected in immersion fixed A549 human lung carcinoma cell line using Goat Anti-Human/Mouse/Rat Peroxiredoxin 5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5724) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm (mitochondria). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Species: Hu, Mu, Rt
Applications: WB, ICC
Catalase Antibody
NBP2-24916
Western Blot: Catalase Antibody [NBP2-24916] - Analysis in liver lysate of 1) human 2) mouse and 3) rat at 0.1 ug/ml.
Immunohistochemistry-Paraffin: Catalase Antibody [NBP2-24916] - Staining of human liver tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.
(1 Review)
(6 Publications)
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC, IHC-P
Thioredoxin-2
Antibody (543317)
MAB5765
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, CH‑1 mouse B cell lymphoma cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat Thioredoxin‑2 Monoclonal Antibody (Catalog # MAB5765) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Thioredoxin‑2 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line and L6 rat myoblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Thioredoxin‑2 at approximately 11 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human/Mouse/Rat Thioredoxin‑2 Monoclonal Antibody (Catalog # MAB5765). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
Species: Hu, Mu, Rt
Applications: WB, Simple Western
Glutaredoxin
3/GLRX3 Antibody
NBP2-20767
Western Blot: Glutaredoxin 3/GLRX3 Antibody [NBP2-20767] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: Glutaredoxin 3/GLRX3 Antibody [NBP2-20767] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: Glutaredoxin 3/GLRX3 Antibody [NBP2-20767] - Human breast carcinoma. TXNL2 antibody [N2C3] dilution: 1:200. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
ASK1 Antibody
[Unconjugated]
AF3575
Western blot shows lysates of Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human ASK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3575) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ASK1 at approximately 154 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
(1 Review)
(2 Publications)
Species: Hu
Applications: WB, ICC
TXNRD2 Antibody
NBP1-86919
Western Blot: TXNRD2 Antibody [NBP1-86919] - Analysis in human cell line K562.
Immunohistochemistry-Paraffin: TXNRD2 Antibody [NBP1-86919] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.
(4 Publications)
Species: Hu
Applications: WB, IHC, IHC-P
Peroxiredoxin 6
Antibody (3A10-2A11)
H00009588-M01
Western Blot: Peroxiredoxin 6 Antibody (3A10-2A11) [H00009588-M01] - PRDX6 monoclonal antibody (M01), clone S1. Analysis of PRDX6 expression in MCF-7.
Western Blot: Peroxiredoxin 6 Antibody (3A10-2A11) [H00009588-M01] - PRDX6 monoclonal antibody (M01), clone 3A10-2A11. Analysis of PRDX6 expression in human liver.
ELISA: Peroxiredoxin 6 Antibody (3A10-2A11) [H00009588-M01] - Detection limit for recombinant GST tagged PRDX6 is 3 ng/ml as a capture antibody.
(11 Publications)
Species: Hu, Bv
Applications: WB, DB, ELISA
TNF-alpha
[Unconjugated]
210-TA
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
Recombinant Human TNF-alpha (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25‑100 pg/mL.
(29 Reviews)
(636 Publications)
Species: Hu
KMT2B Antibody
NB300-864
Immunohistochemistry-Paraffin: KMT2B Antibody [NB300-864] - (5ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: KMT2B Antibody [NB300-864] - (5ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
(1 Publication)
Species: Hu
Applications: IHC, IHC-P, PEP-ELISA
GAPDH Antibody
(1D4)
NB300-221
Western Blot: GAPDH Antibody (1D4) [NB300-221] - analysis of GAPDH in human nucleus pulposus cell culture (40ug/lane) using anti-GAPDH antibody. LI-COR Odyssey imaging system was used for detection. Western blot image submitted by a verified customer review.
Immunocytochemistry/Immunofluorescence: GAPDH Antibody (1D4) [NB300-221] - Analysis of of HeLa cells stained with GAPDH antibody, dilution 1:100 (Green). DAPI staining of nuclear DNA (Blue). The GAPDHantibody produces strong cytoplasmic staining of healthy cells.
Western Blot: GAPDH Antibody (1D4) [NB300-221] - Analysis of cell line lysates probed with mouse mAb to GAPDH (theoretical molecular weight: 36 kDa), NB300-221, dilution 1:2,000: [1] protein standard, [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS1, [6] NIH-3T3, and [7] C6 cells. The GAPDH antibody reveals a single band at ~37 kDa in all cell lines. GAPDH is a house keeping protein, the level of which is relatively unaffected by most experimental manipulations, and, as a result, this antibody has been widely used as a western blotting loading control.
(17 Reviews)
(303 Publications)
Species: Hu, Mu, Rt, Po, Av, Bv, Ca, Ch, ChHa, Eq, Fe, Ha, Ma-Op, Pm, Rb, Sh, Ze
Applications: WB, Simple Western, ChIP, ICC/IF, IHC, IHC-Fr, IP, KD, Single-Cell Western
AKT [p Ser473]
Antibody - Pan Specif
AF887
Western blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 ng/mL Human PDGF (Catalog # 120‑HD) for 15 minutes and MCF‑7 human breast cancer cell line untreated or treated with 100 ng/mL Recombinant Human IGF-1 (Catalog # 291‑G1) for 15 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF887), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-Akt (S473) Pan Specific at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line and A549 human lung carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF‑I (Catalog # 291-G1) for 15 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-Akt (S473) at approximately 60 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF887). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Serum-starved MCF‑7 human breast cancer cells were unstimulated (light orange filled histogram) or activated with 100 ng/mL Recombinant human IGF-1 (Catalog # 291-G1, dark orange filled histogram) for 15 minutes, then stained with Rabbit Anti-Human/Mouse/Rat Phospho‑Akt (S473) Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF887) or control antibody (Catalog # AB-105-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
(23 Publications)
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, CyTOF-ready, ICFlow
GLYAT Antibody
NBP2-58917
Western Blot: GLYAT Antibody [NBP2-58917] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma and human liver tissue.
Immunohistochemistry-Paraffin: GLYAT Antibody [NBP2-58917] - Staining of human skeletal muscle.
Immunohistochemistry-Paraffin: GLYAT Antibody [NBP2-58917] - Staining of human duodenum shows low expression as expected.
Species: Hu
Applications: WB, IHC, IHC-P
Recombinant Human
Glutaredoxin 1/GLRX1 ...
NBC1-22929
Recombinant Human Glutaredoxin 1/GLRX1 Protein
Species: Hu
Applications: PAGE

Publications for Glutaredoxin 1/GLRX1 Recombinant Protein (NBC1-22929) (0)

There are no publications for Glutaredoxin 1/GLRX1 Recombinant Protein (NBC1-22929).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Glutaredoxin 1/GLRX1 Recombinant Protein (NBC1-22929) (0)

There are no reviews for Glutaredoxin 1/GLRX1 Recombinant Protein (NBC1-22929). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for Glutaredoxin 1/GLRX1 Recombinant Protein (NBC1-22929) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

Learn about Custom Antibody Labeling

Additional Glutaredoxin 1/GLRX1 Products

Bioinformatics Tool for Glutaredoxin 1/GLRX1 Recombinant Protein (NBC1-22929)

Discover related pathways, diseases and genes to Glutaredoxin 1/GLRX1 Recombinant Protein (NBC1-22929). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Glutaredoxin 1/GLRX1 Recombinant Protein (NBC1-22929)

Discover more about diseases related to Glutaredoxin 1/GLRX1 Recombinant Protein (NBC1-22929).
 

Pathways for Glutaredoxin 1/GLRX1 Recombinant Protein (NBC1-22929)

View related products by pathway.

PTMs for Glutaredoxin 1/GLRX1 Recombinant Protein (NBC1-22929)

Learn more about PTMs related to Glutaredoxin 1/GLRX1 Recombinant Protein (NBC1-22929).

Blogs on Glutaredoxin 1/GLRX1

There are no specific blogs for Glutaredoxin 1/GLRX1, but you can read our latest blog posts.
Recombinant Monoclonal Antibodies

Customers Who Bought This Also Bought

1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005
Western Blot: GAPDH Antibody (1D4) [NB300-221] - analysis of GAPDH in human nucleus pulposus cell culture (40ug/lane) using anti-GAPDH antibody. LI-COR Odyssey imaging system was used for detection. Western blot image submitted by a verified customer review.
GAPDH Antibody (1D4)
NB300-221

Contact Information

Product PDFs

  PDF Datasheet
  Request CofA
  Request SDS

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human Glutaredoxin 1/GLRX1 Protein and receive a gift card or discount.


Bioinformatics

Gene Symbol GLRX
COVID-19 update