Glutaredoxin 1/GLRX1 Antibody [Unconjugated] Summary
Immunogen |
E. coli-derived recombinant human GLRX1 Met1-Gln106 Accession # P35754 |
Specificity |
Detects human and mouse GLRX1 in Western blots. |
Source |
N/A |
Isotype |
IgG |
Clonality |
Polyclonal |
Host |
Goat |
Gene |
GLRX |
Purity |
Immunogen affinity purified |
Purity Statement |
Antigen Affinity-purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Western Blot 0.5 ug/mL
- Simple Western 5 ug/mL
|
Application Notes |
In Simple Western only 10-15 uL of the recommended dilution is used per data point. |
Packaging, Storage & Formulations
Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
|
Buffer |
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS. |
Preservative |
No Preservative |
Concentration |
LYOPH |
Purity |
Immunogen affinity purified |
Reconstitution Instructions |
Reconstitute at 0.2 mg/mL in sterile PBS. |
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Glutaredoxin 1/GLRX1 Antibody [Unconjugated]
Background
Glutaredoxins or thioltransferases are a universally distributed family of small molecular weight proteins with thiol-disulfide exchange activity.Glutaredoxin 1(GLRX1/GRX) is a 12 kDa protein with glutathione-disulfide oxidoreductase activity. Glutaredoxins utilize the reducing power of glutathione to catalyze disulfide reductions in the presence of NADPH and glutathione reductase (the glutaredoxin system). The amino acid sequence of Glutaredoxin 1 in humans is highly homologous with that in other mammals (81-89%), and contains the conserved active sequence motif -Cys-Pro-Try-Cys-. Glutaredoxin 1 also catalyzes the formation of mixed disulfides (glutathionylation), which is an important redox regulatory mechanism, particularly in mammalian cells under oxidative stress conditions, to sense cellular redox potential.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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