Glutamate Receptor 6 Antibody (1060307) [Alexa Fluor® 594]

• Reactivity: Hu
• Applications: WB
• Clone: 1060307
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 594

Glutamate Receptor 6 Antibody (1060307) [Alexa Fluor® 594] Summary

• Immunogen: Chinese Hamster Ovary cell line CHO-derived human GluR6/GRIK2 protein
  Thr32-Asn549
  Accession # Q13002
• Specificity: Detects recombinant human GluR6/GRIK2 in Western blots.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Glutamate Receptor 6 Antibody (1060307) [Alexa Fluor® 594]

• EAA4
• GLR6
• GluK2
• GLUK6
• GluR6
• glutamate receptor 6
• glutamate receptor, ionotropic, kainate 2
• ionotropic kainate 2
• MGC74427
• MRT6

Background

The Ionotropic Glutamate Receptors are a family of ligand-gated ion channels involved in regulating fast excitatory synaptic transmission in the central nervous system (CNS) (1, 2). The family consists of ~18 members which is divided into 3 groups based on the activating agonist: N-Methyl-D-Aspartate (NMDA), alpha -Amino-3-hydroxy-5-Methyl-4-isoxazole Propionic Acid (AMPA) and kainate (KA) (1, 2). KA receptors (KAR) are found in the pre- and postsynaptic membranes of the central nervous system, most prominently in the hippocampus, where they regulate synaptic transmission, neuronal excitability, and network activity (3). Glutamate receptor 6 (GluR6) (also Glutamate receptor ionotropic, kainate 2 or GluK2) along with GluR5, GluR7 and receptor subunits KA1 and KA2 belong to the KAR sub-group as these molecules are activated by kainic acid (1-3). Mature human GluR6 is a 3-transmembrane protein with a long (530 aa) N-terminal extracellular domain (ECD), a M2 domain involved in pore formation and a short C-terminal intracellular domain (4). The ECD of human GluR6 is highly conserved and shares 99% identity with both mouse and rat. Under physiological conditions, GluR6 forms either a homomeric or heteromeric receptor channel with GluR5 or KA1/2 to become functional (5, 6). Activation of KAR complexes result in Ca2⁺-dependent release of glutamatergic or GABAergic signals (7). The modulatory role of KARs make them attractive therapeutic candidates as glutamate excitotoxicity has been associated with multiple neurodegenerative and mental disorders. Misregulation of GluR6 has been suggested to be a factor in autism, Huntington disease and epilepsy (8). Additionally, GluR6 has been suggested to play a role in tumor suppression in Gastric cancer (9) while potentially displaying the opposite effect in urological cancers (10).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.