Glut4 Antibody

Images

 
Western Blot: Glut4 Antibody [NBP1-49840] - Glucose Transporter GLUT4 expression in bovine skeletal muscle whole lysate. Image from verified customer review.
Western Blot: Glut4 Antibody [NBP1-49840] - Glucose Transporter GLUT4 in Mouse Heart lysate (35ug protein in RIPA buffer) using NBP1-49840 at 1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Glut4 Antibody Summary

Immunogen
Peptide with sequence C-TELEYLGPDEND corresponding to C-Terminus according to NP_001033.1.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SLC2A4
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.3 - 1 ug/ml
  • Peptide ELISA Detection limit 1:16000
Application Notes
WB: Approx. 45 kDa band observed in mouse and rat heart lysates is reported in scientific literature (PMID: 9054401).
Reviewed Applications
Read 1 Review rated 4
using
NBP1-49840 in the following applications:

Publications
Read Publication using NBP1-49840.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Human, Bovine, Porcine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Glut4 Antibody

  • Glut4
  • GLUT-4
  • GLUT4Glucose transporter type 4, insulin-responsive
  • insulin-responsive glucose transporter type 4
  • SLC2A4
  • solute carrier family 2 (facilitated glucose transporter), member 4
  • solute carrier family 2, facilitated glucose transporter member 4

Background

Glucose Transporter 4 (GLUT4/SLC2A4) is a member of a family of proteins that facilitate glucose transport. In response to insulin GLUT4 translocates from low density microsomes to the plasma membrane. Activation of phosphatidylinositol-3-OH kinase is required for this trafficking event but is not sufficient. Insulin resistant subjects with and without diabetes show abnormal accumulation of GLUT4 in a dense membrane compartment in muscle and adipocytes from which insulin is unable to recruit GLUT4 to the cell surface.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Glut4 Antibody (NBP1-49840)(1)

Review for Glut4 Antibody (NBP1-49840) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Other.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-49840:
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Images Ratings Applications Species Date Details
Western Blot Glut4 NBP1-49840
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reviewed by:
Eric England
WB Other 09/30/2014
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Summary

ApplicationWestern Blot
Sample TestedWhole Skeletal Muscle Lysate
SpeciesOther
LotP1
CommentsAntibody is specific, but the protein mw is different than reported. Similar results are seen in porcine samples with this and other antibodies.

Blocking

Blocking Details3% Casein in PBS with 0.1% Tween-20

Primary Anitbody

Dilution Ratio1/2000 at 4C for 18 hr

Secondary Antibody

Secondary DescriptionDonkey anti-Goat IRDye 800CW - Licor Biosciences
Secondary Concentration1/25,000

Details

Detection NotesLicor Odyssey

Comments

CommentsAntibody is specific, but the protein mw is different than reported. Similar results are seen in porcine samples with this and other antibodies.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Glut4 Antibody (NBP1-49840) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Blogs on Glut4.

Glucose Transporter 4 (GLUT4, SLC2A4)
GLUT4 is an insulin-sensitive glucose transporter that facilitates insulin-stimulated glucose uptake in adipose tissue, skeletal muscle, and cardiac tissues that specifically express this protein. It is a twelve transmembrane domain multi-pass protein...  Read full blog post.

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Eric England
09/30/2014
Application: WB
Species: Other

Bioinformatics

Gene Symbol SLC2A4
Entrez
Uniprot