Western Blot: Glut4 Antibody [NBP1-49840] - Glucose Transporter GLUT4 expression in bovine skeletal muscle whole lysate. Image from verified customer review.
Immunohistochemistry-Paraffin: Glut4 Antibody [NBP1-49840] - 3.75ug/ml staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: Glut4 Antibody [NBP1-49840] - Glucose Transporter GLUT4 in Mouse Heart lysate (35ug protein in RIPA buffer) using NBP1-49840 at 1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot: Approx 45kDa band observed in Mouse and Rat Heart lysates (calculated MW of 54.8kDa according to Human NP_001033.1 and Mouse NP_033230.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Fischer et al, J Biol Chem. 1997
Mar 14;272(11):7085-92.; PMID: 9054401). Primary incubation was 1 hour IHC: Paraffin embedded Human Skeletal Muscle.
Read 1 Review rated 4 using NBP1-49840 in the following applications:
Expected from sequence similarity: Bovine, Porcine
Packaging, Storage & Formulations
Store at -20C. Avoid freeze-thaw cycles.
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
0.02% Sodium Azide
Immunogen affinity purified
Alternate Names for Glut4 Antibody
GLUT4Glucose transporter type 4, insulin-responsive
insulin-responsive glucose transporter type 4
solute carrier family 2 (facilitated glucose transporter), member 4
solute carrier family 2, facilitated glucose transporter member 4
Glucose Transporter 4 (GLUT4/SLC2A4) is a member of a family of proteins that facilitate glucose transport. In response to insulin GLUT4 translocates from low density microsomes to the plasma membrane. Activation of phosphatidylinositol-3-OH kinase is required for this trafficking event but is not sufficient. Insulin resistant subjects with and without diabetes show abnormal accumulation of GLUT4 in a dense membrane compartment in muscle and adipocytes from which insulin is unable to recruit GLUT4 to the cell surface.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Glucose Transporter 4 (GLUT4, SLC2A4) GLUT4 is an insulin-sensitive glucose transporter that facilitates insulin-stimulated glucose uptake in adipose tissue, skeletal muscle, and cardiac tissues that specifically express this protein. It is a twelve transmembrane domain multi-pass protein... Read full blog post.
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