Glut4 Antibody (925951) [Unconjugated]

Glut4 was detected in immersion fixed human mesenchymal stem cells differentiated to adipocytes using Mouse Anti-Human Glut4 Monoclonal Antibody (Catalog # MAB8654) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Glut4 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human Glut4 Monoclonal Antibody (Catalog # MAB8654) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in tubular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: IHC, ICC/IF
• Clone: 925951
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Glut4 Antibody (925951) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Glut4
  Met1-Asp509
  Accession # P14672
• Specificity: Detects human Glut4 in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: SLC2A4
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Glut4 Antibody (925951) [Unconjugated]

• Glut4
• GLUT-4
• GLUT4Glucose transporter type 4, insulin-responsive
• insulin-responsive glucose transporter type 4
• SLC2A4
• solute carrier family 2 (facilitated glucose transporter), member 4
• solute carrier family 2, facilitated glucose transporter member 4

Background

Glut4 (Glucose Transporter Member 4), also known as SLC2A4, is a 509 aa multi-pass type membrane protein and shares 65% aa identity with mouse Glut4.  It is an insulin-regulated glucose transporter.  Glut4 is most highly expressed in adipose and striated muscle tissues, but has also been reported in multiple other tissues including the nervous system and breast cancer.  Insulin stimulated transport of Glut4 has been shown to be impaired in type 2 diabetes patients.  Additionally, inhibition of Glut4 may be useful in reducing proliferation of breast cancer cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Glut4 Antibody (MAB8654)(1)

Publication using MAB8654

• Jonas R Knudsen, Kaspar W Persson, Carlos Henriquez-Olguin, Zhencheng Li, Nicolas Di Leo, Sofie A Hesselager et al. Microtubule-mediated GLUT4 trafficking is disrupted in insulin-resistant skeletal muscle eLife 2023-04-19 [PMID: 37073948]

Bioinformatics

• Gene Symbol: SLC2A4
• Uniprot:
  • P14672()