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Glut4 Antibody (2E11NB) [Janelia Fluor® 646]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P
Clone
2E11NB
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 646

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-44299AF350
Alexa Fluor 405
NBP2-44299AF405
Alexa Fluor 488
NBP2-44299AF488
Alexa Fluor 532
NBP2-44299AF532
Alexa Fluor 594
NBP2-44299AF594
Alexa Fluor 647
NBP2-44299AF647
Alexa Fluor 700
NBP2-44299AF700
Alexa Fluor 750
NBP2-44299AF750
Biotin
NBP2-44299B
DyLight 350
NBP2-44299UV
DyLight 405
NBP2-44299V
DyLight 488
NBP2-44299G
DyLight 550
NBP2-44299R
DyLight 594
NBP2-44299DL594
DyLight 650
NBP2-44299C
DyLight 680
NBP2-44299FR
DyLight 755
NBP2-44299IR
FITC
NBP2-44299F
HRP
NBP2-44299H
Janelia Fluor 549
NBP2-44299JF549
Unconjugated
NBP2-44299
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-44299
Azide and BSA Free
NBP2-80754
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Glut4 Antibody (2E11NB) [Janelia Fluor® 646] Summary

Immunogen
Partial recombinant human protein (between amino acids 300-350) [UniProt# P14672]
Localization
Cell Membrane, ER Membrane, Perinuclear
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SLC2A4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Glut4 Antibody (2E11NB) [Janelia Fluor® 646]

  • Glut4
  • GLUT-4
  • GLUT4Glucose transporter type 4, insulin-responsive
  • insulin-responsive glucose transporter type 4
  • SLC2A4
  • solute carrier family 2 (facilitated glucose transporter), member 4
  • solute carrier family 2, facilitated glucose transporter member 4

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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(E2-E3 (same as INS04 ...
NBP2-34260
Western Blot: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Probing of Western blots of pancreatic lysates from control and iron overloaded Tg40 PrP and PrP-/- mice with antibodies specific for insulin dimer and pentamer (upper and lower panels) shows decreased expression in Tg40 PrP relative to PrP-/- samples (lanes 1 & 5 vs. 3 & 7). Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-24786-1), licensed under a CC-BY licence.
Immunohistochemistry: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Immunohistochemistry of pancreas shows relatively higher reactivity for insulin in PrP-/- relative to Tg40 PrP sections (panels 1 & 3). Iron overloading decreases insulin reactivity in Tg40 PrP but not in PrP-/- samples (panels 1 vs. 2 & 3 vs. 4). Reaction for glucagon is higher in iron overloaded Tg40 PrP relative to control samples (panels 5 & 6). The difference is minimal in PrP-/- samples (panels 7 & 8). Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41598-018-24786-1), licensed under a CC-BY licence.
Immunohistochemistry-Paraffin: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Formalin-fixed, paraffin-embedded human pancreas stained with insulin Monoclonal Antibody (E2-E3)
(5 Reviews)
(1 Publication)
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Glut1 Antibody
NB110-39113
Western Blot: Glut1 Antibody [NB110-39113] - PrP-mediated increase in IC iron downregulates glucose transporters in the brain, neuroretina, and the liver: A similar evaluation of retinal lysates shows upregulation of Glut1 in PrP-/- relative to Tg40 PrP samples (lanes 1 & 3). Iron overloading downregulates Glut1 in Tg40 PrP samples relative to untreated controls, but has minimal effect on similarly treated PrP-/- samples (lanes 2 & 4). Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41598-018-24786-1) licensed under a CC-BY licence.
Western Blot: Glut1 Antibody [NB110-39113] - Western blot of GLUT1 on mouse kidney membrane protein (lane A) and rat kidney membrane protein (lane B).
Immunohistochemistry-Paraffin: Glut1 Antibody [NB110-39113] - Immunohistochemical analysis of FFPE tissue section of human placenta using 1:200 dilution of Glut1 antibody. The staining was developed using HRP-DAB detection method and the sections were further counterstained with hematoxylin. This antibody generated a specific strong membrane cytoplasmic staining of Glut1 primarily in the syncytiotrophoblast layers of various villi and in the red blood cells (RBCs). Cytotrophoblasts showed a very weak expression of this protein.
(7 Reviews)
(40 Publications)
Species: Hu, Mu, Rt, Rb
Applications: WB, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, Flow-IC
AKT [p Ser473]
Antibody - Pan Specif
AF887
Western blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 ng/mL Human PDGF (Catalog # 120‑HD) for 15 minutes and MCF‑7 human breast cancer cell line untreated or treated with 100 ng/mL Recombinant Human IGF-1 (Catalog # 291‑G1) for 15 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF887), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-Akt (S473) Pan Specific at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line and A549 human lung carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF‑I (Catalog # 291-G1) for 15 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-Akt (S473) at approximately 60 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF887). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Serum-starved MCF‑7 human breast cancer cells were unstimulated (light orange filled histogram) or activated with 100 ng/mL Recombinant human IGF-1 (Catalog # 291-G1, dark orange filled histogram) for 15 minutes, then stained with Rabbit Anti-Human/Mouse/Rat Phospho‑Akt (S473) Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF887) or control antibody (Catalog # AB-105-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
(23 Publications)
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, CyTOF-ready, ICFlow
IRS1 Antibody
NB100-82001
Western Blot: IRS1 Antibody [NB100-82001] - Analysis of extracts from 293 cells using IRS-1 (Ab-636) antibody
Immunocytochemistry/Immunofluorescence: IRS1 Antibody [NB100-82001] - Staining of methanol-fixed Hela cells using IRS-1 (Ab-636) antibody
Immunohistochemistry-Paraffin: IRS1 Antibody [NB100-82001] - Analysis of breast carcinoma tissue, using IRS-1 (Ab-636) antibody.
(3 Publications)
Species: Hu, Mu, Rt, Po
Applications: WB, ICC/IF, IHC, IHC-P
Insulin Receptor
beta Antibody
NBP2-12793
Immunohistochemistry-Paraffin: Insulin Receptor beta Antibody [NBP2-12793] - Staining shows expression of insulin receptor beta in the Cortex in mouse brain. Dilution 1:500
Immunoprecipitation: Insulin Receptor beta Antibody [NBP2-12793] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), Jurkat (J; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Insulin Receptor Beta antibody NBP2-12793 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. Insulin Receptor Beta was also immunoprecipitated by rabbit anti-Insulin Receptor Beta antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).
(1 Review)
(2 Publications)
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IP
Leucyl-cystinyl
Aminopeptidase/LNPEP ...
AF6386
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Human Leucyl-cystinyl Aminopeptidase/LNPEP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6386) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Leucyl-cystinyl Aminopeptidase/LNPEP at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Species: Hu
Applications: WB, IP
PYK2/FAK2 Antibody
(SC06-15)
NBP2-67627
Western Blot: PYK2/FAK2 Antibody (SC06-15) [NBP2-67627] - Analysis of PYK2 on mouse brain lysates using anti-PYK2 antibody at 1:1000 dilution.
Immunocytochemistry/Immunofluorescence: PYK2/FAK2 Antibody (SC06-15) [NBP2-67627] - Staining PYK2 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X-100/PBS.
Immunohistochemistry-Paraffin: PYK2/FAK2 Antibody (SC06-15) [NBP2-67627] - Analysis of paraffin-embedded mouse kidney tissue using anti-PYK2 antibody. Counter stained with hematoxylin.
(1 Review)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
PPAR gamma/NR1C3
Antibody
NBP2-22106
Western Blot: PPAR gamma/NR1C3 Antibody [NBP2-22106] - Total protein from 3T3-L1 mouse embryonic fibroblast adipose-like cell line, separated by 4-12% SDS-PAGE, transferred to nitrocellulose membrane and blocked in 5% non-fat milk for 1h at room temperature. The membrane was probed with anti-PPAR gamma 1:800 in non-fat milk. Lane 2-7 increasing protein concentration, undifferentiated adipocytes. Lane 8-13 increasing protein concentration, differentiated adipocytes. This image was submitted via customer review.
Immunohistochemistry-Paraffin: PPAR gamma Antibody [NBP2-22106] - IHC analysis of PPAR gamma in mouse liver.
Western Blot: PPAR gamma Antibody [NBP2-22106] - Western blot analysis of PPAR gamma in 1. human adipose and 2. human adrenal lysate.
(2 Reviews)
(5 Publications)
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P, IP
Glut3 Antibody
(JA50-31)
NBP2-66872
Western Blot: Glut3 Antibody (JA50-31) [NBP2-66872] - Analysis of Glucose Transporter GLUT3 on different lysates using anti-Glucose Transporter GLUT3 antibody at 1/1,000 dilution. Positive control: Lane1: Human lung Lane2: HepG2 Lane3: Mouse heart
Immunohistochemistry-Paraffin: Glut3 Antibody (JA50-31) [NBP2-66872] - Analysis of paraffin-embedded mouse brain tissue using anti-Glucose Transporter GLUT3 antibody. Counter stained with hematoxylin.
Flow Cytometry: Glut3 Antibody (JA50-31) [NBP2-66872] - Analysis of SH-SY5Y cells with Glucose Transporter GLUT3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
(2 Publications)
Species: Hu, Mu, Rt
Applications: WB, Flow, IHC, IHC-P
TNF-alpha
[Unconjugated]
210-TA
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
Recombinant Human TNF-alpha (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25‑100 pg/mL.
(33 Reviews)
(647 Publications)
Species: Hu
AMPK alpha 2
Antibody [Unconjugate
AF2850
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and mouse liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat AMPK alpha 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2850) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for AMPK alpha 2 at approximately 63 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for AMPK alpha 2 at approximately 63 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat AMPK alpha 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2850) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
AMPK alpha 2 was detected in immersion fixed HEK293 human embryonic kidney cell line using Goat Anti-Human/Mouse/Rat AMPK alpha 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2850) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
(1 Review)
(5 Publications)
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, ICC
AMPK alpha 1
Antibody (2B7)
NBP2-22127
Western Blot: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - Ability to combat ROS varies across cell lines. Western blot analysis showing levels of AMPK and activated AMPK (T172) at 24, 48 and 72 hours following exposure to 0.4 uM GNE-617. Full-length western blots are shown in S2D Fig. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0164166), licensed under a CC-BY licence.
Immunocytochemistry/Immunofluorescence: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - Immunofluorescence analysis of NTERA-2 cells using AMPK alpha 1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - Immunohistochemical analysis of paraffin-embedded ovarian cancer (left) and brain tissues (right) using AMPK alpha 1 mouse mAb with DAB staining.
(9 Publications)
Species: Hu, Mu, Rt, Pm
Applications: WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-P
AMPK beta 1
Antibody (2C3)
H00005564-M02
Western Blot: AMPK beta 1 Antibody (2C3) [H00005564-M02] - PRKAB1 monoclonal antibody (M02), clone 2C3 Analysis of PRKAB1 expression in HeLa.
Immunocytochemistry/Immunofluorescence: AMPK beta 1 Antibody (2C3) [H00005564-M02] - Analysis of monoclonal antibody to PRKAB1 on HeLa cell. Antibody concentration 10 ug/ml.
Sandwich ELISA: AMPK beta 1 Antibody (2C3) [H00005564-M02] - Detection limit for recombinant GST tagged PRKAB1 is approximately 0.1ng/ml as a capture antibody.
(1 Review)
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, S-ELISA
TM4SF1/L6 Antibody
NBP1-76549
Western Blot: TM4SF1/L6 Antibody [NBP1-76549] - Human lung tissue lysate with TM4SF1 antibody at (A) 0.5 and (B) 1 ug/mL.
Immunohistochemistry-Paraffin: TM4SF1/L6 Antibody [NBP1-76549] - Staining of TM4SF1 in mouse lung tissue with TM4SF1 antibody at 5 ug/mL.
Immunohistochemistry-Paraffin: TM4SF1/L6 Antibody [NBP1-76549] - Mouse lung tissue with TM4SF1 antibody at 2 ug/mL.
Species: Hu, Mu, Rt
Applications: WB, ELISA, IHC, IHC-P, IF
RPL6 Antibody
NBP2-20216
Western Blot: RPL6 Antibody [NBP2-20216] - Sample (30 ug of whole cell lysate) A: A549 B: H1299 C: HCT116 D: MCF-7 12% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: RPL6 Antibody [NBP2-20216] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: RPL6 Antibody [NBP2-20216] - Paraffin-embedded rat fore brain. RPL6 antibody dilution: 1:500.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
MED10 Antibody
NBP1-81328
Western Blot: MED10 Antibody [NBP1-81328] - Analysis in control (vector only transfected HEK293T lysate) and MED10 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/Immunofluorescence: MED10 Antibody [NBP1-81328] - Staining of human cell line PC-3 shows localization to nucleoplasm. Antibody staining is shown in green.
Species: Hu
Applications: WB, ICC/IF
TBC1D4 Antibody
(OTI5E6)
NBP2-00597
Western Blot: TBC1D4 Antibody (5E6) [NBP2-00597] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TBC1D4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TBC1D4.
Immunocytochemistry/Immunofluorescence: TBC1D4 Antibody (OTI5E6) [NBP2-00597] - TBC1D4 Antibody (5E6) [NBP2-00597] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY TBC1D4.
Flow Cytometry: TBC1D4 Antibody (5E6) [NBP2-00597] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TBC1D4 antibody, and then analyzed by flow cytometry.
Species: Hu
Applications: WB, Flow, ICC/IF
Adiponectin/Acrp30
[HRP]
DRP300
(3 Reviews)
(117 Publications)
Species: Hu

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Product General Protocols

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-44299AF350
Alexa Fluor 405 NBP2-44299AF405
Alexa Fluor 488 NBP2-44299AF488
Alexa Fluor 532 NBP2-44299AF532
Alexa Fluor 594 NBP2-44299AF594
Alexa Fluor 647 NBP2-44299AF647
Alexa Fluor 700 NBP2-44299AF700
Alexa Fluor 750 NBP2-44299AF750
Biotin NBP2-44299B
DyLight 350 NBP2-44299UV
DyLight 405 NBP2-44299V
DyLight 488 NBP2-44299G
DyLight 550 NBP2-44299R
DyLight 594 NBP2-44299DL594
DyLight 650 NBP2-44299C
DyLight 680 NBP2-44299FR
DyLight 755 NBP2-44299IR
FITC NBP2-44299F
HRP NBP2-44299H
Janelia Fluor 549 NBP2-44299JF549
Janelia Fluor 646 NBP2-44299JF646
Learn about Custom Antibody Labeling

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Blogs on Glut4.

Check out the latest blog posts on Glut4.
Glucose Transporter 4 (GLUT4, SLC2A4)
GLUT4 is an insulin-sensitive glucose transporter that facilitates insulin-stimulated glucose uptake in adipose tissue, skeletal muscle, and cardiac tissues that specifically express this protein. It is a twelve transmembrane domain multi-pass protein...  Read full blog post.
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Bioinformatics

Gene Symbol SLC2A4