Glucagon Antibody (09) [Alexa Fluor® 647]

Product Image: Glucagon Antibody (09) [Alexa Fluor® 647] [NBP2-21803AF647] - Vial of Alexa Fluor 647 conjugated antibody. Alexa Fluor 647 is optimally excited at 653 nm by the Red laser (633 or 640 nm) and has an emission maximum of 669 nm.

• Reactivity: Hu, Mu, Rt, Po
• Applications: ELISA, Flow, ICC/IF, IHC, ELISA
• Clone: 09
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 647

Glucagon Antibody (09) [Alexa Fluor® 647] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: Synthetic full length oxyntomodulin coupled to carrier.
• Epitope: The epitope lies within the C-terminal region of glucagon.
• Specificity: Binds human glucagon. Cross-reacts less than 5% with oxyntomodulin.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: GCG
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA Capture
  • Sandwich ELISA
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product NBP2-21803. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:32953935).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Glucagon Antibody (09) [Alexa Fluor® 647]

• GCG
• glicentin-related polypeptide
• GLP1
• GLP-1
• Glucagon
• glucagon-like peptide 1
• glucagon-like peptide 2
• GRPP

Background

The protein encoded by the Glucagon gene is actually a preproprotein that is cleaved into four distinct mature peptides. One ofthese, glucagon, is a pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulatingglycogenolysis and gluconeogenesis. Glucagon is a ligand for a specific G-protein linked receptor whose signallingpathway controls cell proliferation. Two of the other peptides are secreted from gut endocrine cells and promotenutrient absorption through distinct mechanisms. Finally, the fourth peptide is similar to glicentin, an activeenteroglucagon. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Glucagon Antibody (NBP2-21803AF647)(4)

Publications using NBP2-21803AF647

• Xiaomeng Hu, Kathy White, Chi Young, Ari G Olroyd, Paul Kievit, Andrew J Connolly, Tobias Deuse, Sonja Schrepfer Hypoimmune islets achieve insulin independence after allogeneic transplantation in a fully immunocompetent non-human primate. Cell stem cell 2024-03-11 [PMID: 38335966]
• Wu Y, Wong CW, Chiles EN Et al. Glycerate from intestinal fructose metabolism induces islet cell damage and glucose intolerance Cell Metab 2022-06-10 [PMID: 35688154]
  
  Details:
  Citation using the Alexa Fluor 647 version of this antibody.
• Lau H, Corrales N, Rodriguez S et al. Dose-dependent effects of necrostatin-1 supplementation to tissue culture media of young porcine islets PloS one 2020-12-07 [PMID: 33284818] (Western Blot, Human)
• Lau, H, Corrales, N Et al. An islet maturation media to improve the development of young porcine islets during in vitro culture. Islets 2020-05-03 [PMID: 32459554] (WB, Human)

Bioinformatics

• Gene Symbol: GCG