GLP1 Antibody (4F3) - BSA Free

Glucagon Antibody (4F3) - BSA Free [NBP1-97413] - BSA Free [NBP1-97413]

ELISA: Glucagon Antibody (4F3) - BSA Free [NBP1-97413] - This uses NBP2-23506 as capture antibody in combination with biotinylated detection antibody NBP1-97413 to measure the sum of GLP-1(7-36)amide, GLP-1(1-36)amide and GLP-1(9-36)amide in biological samples. Relative to measuring the two active forms of GLP-1, the responses are reduced by not measuring the contribution of GLP-1(7- 37) to the overall response, but this is compensated for by measuring an approximately equal contribution from GLP-1(1-36)amide. However, the GLP-1(9-36)amide degradation product is also measured, augmenting the response above that of the active forms alone. This assay cannot detect changes in active GLP-1 in response to treatment with DPP-4 inhibitors, and is therefore only suitable for experiments in which changes in GLP-1 degradation are irrelevant.

Product Discontinued

GLP1 Antibody (4F3) - BSA Free Summary

• Immunogen: Synthetic GLP-1(7-36)amide coupled to carrier and adsorbed onto aluminum hydroxide gel.
• Epitope: Mid-molecular epitope of GLP-1
• Specificity: Reacts with all forms of GLP-1, including precursor and GLP-1(9-37) /GLP-1(9-36amide) metabolite.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: GCG
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry
  • Western Blot 1:2000
• Application Notes: NBP1-97413 binds to GLP-1 when coated directly onto the microtiter well, and binds GLP-1(7-36)amide in solution giving a Ka of 4.0x10E8 in inhibition ELISA. NBP1-97413 cross-reacts <0.4% with coated glucagon. In inhibition ELISA no binding of free glucagon in solution is detected, giving an estimated cross-reactivity of <0.2%. Although not tested, NBP1-97413 is likely to detect all known molecular forms of GLP-1 in immunohistochemistry.
  NBP1-97413 can be used as a capture antibody in combination with NBP1-20487 as a detection antibody for measuring non-amidated GLP-1 forms and cross- reacting about 16% with C-terminally amidated GLP-1. Results show detection limits of 44pmol/L which is 10-20 times higher than the basal concentration of GLP-1, so the assays have to be optimized.

Reactivity Notes

Human.The sequence of GLP-1 is fully conserved in all mammalian species examined so far.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 10mM Phosphate (pH 7.4) and 0.5M NaCl
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for GLP1 Antibody (4F3) - BSA Free

• glicentin-related polypeptide
• GLP1
• GLP-1
• glucagon-like peptide 1
• GRPP

Background

Glucagon-like peptide-1(7-36)amide (GLP-1(7-36)amide) is the principal active form of GLP-1, the other being GLP-1(7-37). GLP-1 is a peptide hormone of the glucagon family, produced by the L cells of the intestinal mucosa from the same prohormone as glucagon. The active forms are potent stimulators of glucose-dependent insulin secretion. The sequence of GLP-1 is fully conserved in all mammalian species examined so far.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GCG