GLP1 Antibody (11E2) - BSA Free

Glucagon Antibody (11E2) - BSA Free [NBP2-23561]

• Reactivity: Hu, Ma
• Applications: WB, ELISA
• Clone: 11E2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

GLP1 Antibody (11E2) - BSA Free Summary

• Immunogen: Synthetic GLP-1(7-36)amide coupled to carrier and adsorbed onto aluminum hydroxide gel
• Epitope: Mid-molecular epitope of GLP-1
• Specificity: Reacts with all forms of GLP-1, including precursor and GLP-1(9-37) /GLP-1(9-36amide) metabolite
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: GCG
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Western Blot 1:100-1:2000
• Application Notes: Clone 11E2 binds to GLP-1 when coated directly onto the microtiter well, and binds GLP-1(7-36)amide in solution giving a Ka of 1.2x10E7 in inhibition ELISA. Clone 11E2 does not cross-react with coated glucagon or hGLP-2. In inhibition ELISA no binding of free glucagon in solution is detected, giving an estimated cross-reactivity of <0.2%. Although not tested, Clone 11E2 is likely to detect all known molecular forms of GLP-1 in immunohistochemistry. In Western blotting a dilution guideline of 1/1000 has proved successful.

Reactivity Notes

Mammal

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 10mM Phosphate (pH 7.4) and 0.5M NaCl
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for GLP1 Antibody (11E2) - BSA Free

• glicentin-related polypeptide
• GLP1
• GLP-1
• glucagon-like peptide 1
• GRPP

Background

Glucagon-like peptide 1(7-36)amide (GLP-1(7-36)amide) is the principal active form of GLP-1, the other being GLP-1(7-37). GLP-1 is a peptide hormone of the glucagon family, produced by the L cells of the intestinal mucosa from the same prohormone as glucagon. The active forms are potent stimulators of glucose-dependent insulin secretion. The sequence of GLP-1 is fully conserved in all mammalian species examined so far.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GCG