GLP/EHMT1 Antibody (1H2) - Azide and BSA Free

Western Blot: GLP/EHMT1 Antibody (1H2) [H00079813-M04] - Analysis of EHMT1 expression in MES-SA/Dx5 (Cat # L021V1).

Immunocytochemistry/Immunofluorescence: GLP/EHMT1 Antibody (1H2) [H00079813-M04] - Analysis of monoclonal antibody to EHMT1 on HeLa cell. Antibody concentration 10 ug/ml

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 1H2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

GLP/EHMT1 Antibody (1H2) - Azide and BSA Free Summary

• Immunogen: EHMT1 (NP_079033, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAADEGSAEKQAGEAHMAADGETNGSCENSDASSHANAAKHTQDSARVNPQDGTNTLTRIAENGVSERDSEAAKQNHVTADDFVQTSVIGSNGYILNKPA
• Specificity: EHMT1 - euchromatic histone-lysine N-methyltransferase 1 (1H2)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: EHMT1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for GLP/EHMT1 Antibody (1H2) - Azide and BSA Free

• DEL9q34
• EC 2.1.1.-
• EC 2.1.1.43
• EHMT1
• euchromatic histone-lysine N-methyltransferase 1H3-K9-HMTase 5
• Eu-HMTase1
• Eu-HMTase1DEL9q34
• EUHMTASE1FP13812
• FLJ12879
• G9a-like protein 1
• GLP
• GLP1
• H3 lysine-9 specific 5
• KIAA1876euchromatic histone methyltransferase 1
• KMT1D
• KMT1DDKFZp667M072
• Lysine N-methyltransferase 1D

Background

Euchromatic histone-lysine N-methyltransferase 1 (EHMT1) is a histone methyltransferase that catalyzes the methylation of lysine-9 of histone H3 (H3-K9). H3-K9 histone methylation is restricted to euchromatin and functions to epigenetically silence gene transcription. Loss of function mutations in EHMT1 cause the 9q34 subtelomeric deletion syndrome, a syndrome characterized by severe mental retardation, hypotonia, brachy(micro)cephaly, heart defects, and distinct facial anomalies. Alternate names for EHMT1 include histone H3-K9 methyltransferase 5, H3-K9-HMTase5, eu-HMTase, G9a-like protein 1, GLP1, lysine N-methyltransferase 1D, EUHMTASE1, KMT1D, and KIAA1876.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EHMT1
• Entrez:
  • 79813(Human)
• Uniprot:
  • NP_079033(Human)