GLE1 Antibody

Western Blot: GLE1 Antibody [NBP1-32714] - Sample (30 ug of whole cell lysate) A: HeLa 7.5% SDS PAGE, antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: GLE1 Antibody [NBP1-32714] - Paraformaldehyde-fixed HeLa, using GLE1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2500.

Immunohistochemistry-Paraffin: GLE1 Antibody [NBP1-32714] - GLE1 antibody detects GLE1 protein at cytoplasm and nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. GLE1 antibody diluted at 1:500.

Immunohistochemistry-Paraffin: GLE1 Antibody [NBP1-32714] - Paraffin-embedded Cal27 xenograft, using antibody at 1:500 dilution.

Product Discontinued

GLE1 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human GLE1. The exact sequence is proprietary.
• Localization: Nucleus, Cytoplasm, Isoform 1: Cytoplasm, nuclear pore complex
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GLE1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for GLE1 Antibody

• GLE1 (yeast homolog)-like, RNA export mediator
• GLE1 RNA export mediator homolog (yeast)
• GLE1LGLE1 RNA export mediator-like (yeast)
• GLE1-like protein
• GLE1-like, RNA export mediator
• hGLE1GLE1 RNA export mediator (yeast)
• LCCS
• LCCS1
• lethal congenital contracture syndrome 1
• nucleoporin GLE1

Background

This gene encodes a predicted 75-kDa polypeptide with high sequence and structure homology to yeast Gle1p, which is nuclear protein with a leucine-rich nuclear export sequence essential for poly(A)+RNA export. Inhibition of human GLE1L by microinjection of antibodies against GLE1L in HeLa cells resulted in inhibition of poly(A)+RNA export. Immunofluorescence studies show that GLE1L is localized at the nuclear pore complexes. This localization suggests that GLE1L may act at a terminal step in the export of mature RNA messages to the cytoplasm. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: GLE1