GLE1 Antibody

Images

 
Western Blot: GLE1 Antibody [NBP1-32714] - Sample (30 ug of whole cell lysate) A: HeLa 7.5% SDS PAGE, antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: GLE1 Antibody [NBP1-32714] - Paraformaldehyde-fixed HeLa, using GLE1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) ...read more
Immunohistochemistry-Paraffin: GLE1 Antibody [NBP1-32714] - Paraffin-embedded Cal27 xenograft, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

GLE1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human GLE1. The exact sequence is proprietary.
Localization
Nucleus, Cytoplasm, Isoform 1: Cytoplasm, nuclear pore complex
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GLE1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for GLE1 Antibody

  • GLE1 (yeast homolog)-like, RNA export mediator
  • GLE1 RNA export mediator homolog (yeast)
  • GLE1LGLE1 RNA export mediator-like (yeast)
  • GLE1-like protein
  • GLE1-like, RNA export mediator
  • hGLE1GLE1 RNA export mediator (yeast)
  • LCCS
  • LCCS1
  • lethal congenital contracture syndrome 1
  • nucleoporin GLE1

Background

This gene encodes a predicted 75-kDa polypeptide with high sequence and structure homology to yeast Gle1p, which is nuclear protein with a leucine-rich nuclear export sequence essential for poly(A)+RNA export. Inhibition of human GLE1L by microinjection of antibodies against GLE1L in HeLa cells resulted in inhibition of poly(A)+RNA export. Immunofluorescence studies show that GLE1L is localized at the nuclear pore complexes. This localization suggests that GLE1L may act at a terminal step in the export of mature RNA messages to the cytoplasm. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GLE1