GKAP/DLGAP1 Antibody (3G4)

Western Blot: GKAP/DLGAP1 Antibody (3G4) [NBP2-37582] - Western blot analysis using GKAP mAb against HEK293 (1) and GKAP(AA: 490-663)-hIgGFc transfected HEK293 (2) cell lysate.

Immunocytochemistry/Immunofluorescence: GKAP/DLGAP1 Antibody (3G4) [NBP2-37582] - Immunofluorescence analysis of NIH/3T3 cells using GKAP mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunohistochemistry: GKAP/DLGAP1 Antibody (3G4) [NBP2-37582] - Immunohistochemical analysis of paraffin-embedded human esophagus tissues using GKAP mouse mAb with DAB staining.

Immunohistochemistry: GKAP/DLGAP1 Antibody (3G4) [NBP2-37582] - Immunohistochemical analysis of paraffin-embedded human liver cancer tissues using GKAP mouse mAb with DAB staining.

ELISA: GKAP/DLGAP1 Antibody (3G4) [NBP2-37582] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Discontinued

GKAP/DLGAP1 Antibody (3G4) Summary

• Immunogen: Purified recombinant fragment of human GKAP expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: DLGAP1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 109 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for GKAP/DLGAP1 Antibody (3G4)

• DAP1
• DAP-1-ALPHA
• DAP-1DAP-1-BETA
• discs, large (Drosophila) homolog-associated protein 1
• disks large-associated protein 1
• DLGAP1
• GKAP
• GKAPMGC88156
• hGKAP
• PSD-95/SAP90 binding protein 1
• PSD-95/SAP90-binding protein 1
• SAP90/PSD-95-associated protein 1
• SAPAP1
• SAPAP1Guanylate kinase-associated protein

Background

Members of the postsynaptic density-95 (PSD-95)/SAP90 family of membraneassociated guanylate kinase (MAGUK) proteins function as multimodular scaffolds that organize protein-signaling complexes at neuronal synapses. PSD-95/SAP90 binds guanylate kinase-associated protein (GKAP), also designated GK domain-binding protein, DAP-1-alpha, DAP-1-beta, PSD-95 binding protein, PSD-95/SAP90 associated protein, or SAPAP1, through the guanylate kinase domain. SAPAP1 is expressed widely in neurons of the cortex and hippocampus and in the Purkinje and granule cells of the cerebellum. N-terminal splice variants of SAPAP1 are expressed as 95 and 130 kDa proteins. SAPAP1 is localized specifically in the PSD of glutamatergic synapses, consistent with its direct interaction with PSD-95 family proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DLGAP1