GIT2 Antibody

Western Blot: GIT2 Antibody [NBP3-13188] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GIT2 antibody (NBP3-13188) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: GIT2 Antibody [NBP3-13188] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GIT2 antibody (NBP3-13188) diluted at 1:1000.

Western Blot: GIT2 Antibody [NBP3-13188] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GIT2 antibody (NBP3-13188) diluted at 1:1000.

Western Blot: GIT2 Antibody [NBP3-13188] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GIT2 antibody (NBP3-13188) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

GIT2 Antibody Summary

• Description: Centrifuge briefly prior to opening.
• Immunogen: Recombinant protein encompassing a sequence within the center region of mouse GIT2. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GIT2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot

Reactivity Notes

Human (85%), Rhesus Monkey (85%), Bovine (83%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for GIT2 Antibody

• ARF GAP GIT2
• ARF GTPase-activating protein GIT2
• CAT2
• CAT-2
• cool-associated, tyrosine phosphorylated protein 2
• Cool-interacting tyrosine-phosphorylated protein 2
• DKFZp686G01261
• G protein-coupled receptor kinase interacting ArfGAP 2
• G protein-coupled receptor kinase interactor 2
• G protein-coupled receptor kinase-interactor 2
• KIAA0148GRK-interacting protein 2
• MGC760

Background

GIT2 encodes a member of the GIT protein family. GIT proteins interact with G protein-coupled receptor kinases and possess ADP-ribosylation factor (ARF) GTPase-activating protein (GAP) activity. This gene undergoes extensive alternative splicing; although ten transcript variants have been described, the full length sequence has been determined for only four variants. The various isoforms have functional differences, with respect to ARF GAP activity and to G protein-coupled receptor kinase 2 binding.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GIT2