GIT2 Antibody - BSA Free

Immunohistochemistry: GIT2 Antibody [NBP1-19143] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-GIT2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Immunohistochemistry-Paraffin: GIT2 Antibody [NBP1-19143] - FFPE section of human lung carcinoma. Affinity purified rabbit anti-GIT2 used at a dilution of 1:500.

Immunoprecipitation: GIT2 Antibody [NBP1-19143] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. NBP1-19143 used for IP at 3 mcg/mg lysate. GIT2 was also immunoprecipitated by NBP1-19144 which recognize downstream epitopes.

• Reactivity: Hu
• Applications: IHC, IP, WB (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

GIT2 Antibody - BSA Free Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 500 and 550 of human G protein-coupled receptor kinase interacting ArfGAP 2 using the numbering given in entry NP_476510.1 (GeneID 9815).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GIT2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:1000-1:5000
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GIT2 Antibody - BSA Free

• ARF GAP GIT2
• ARF GTPase-activating protein GIT2
• CAT2
• CAT-2
• cool-associated, tyrosine phosphorylated protein 2
• Cool-interacting tyrosine-phosphorylated protein 2
• DKFZp686G01261
• G protein-coupled receptor kinase interacting ArfGAP 2
• G protein-coupled receptor kinase interactor 2
• G protein-coupled receptor kinase-interactor 2
• KIAA0148GRK-interacting protein 2
• MGC760

Background

This gene encodes a member of the GIT protein family, which interact with G protein-coupled receptor kinases and possess ADP-ribosylation factor (ARF) GTPase-activating protein (GAP) activity. GIT proteins traffic between cytoplasmic complexes, focal adhesions, and the cell periphery, and interact with Pak interacting exchange factor beta (PIX) to form large oligomeric complexes that transiently recruit other proteins. GIT proteins regulate cytoskeletal dynamics and participate in receptor internalization and membrane trafficking. This gene has been shown to repress lamellipodial extension and focal adhesion turnover, and is thought to regulate cell motility. This gene undergoes extensive alternative splicing to generate multiple isoforms, but the full-length nature of some of these variants has not been determined. The various isoforms have functional differences, with respect to ARF GAP activity and to G protein-coupled receptor kinase 2 binding. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GIT2
• Entrez:
  • 9815(Human)
• Uniprot:
  • NP_476510.1(Human)