GGA1 Antibody - BSA Free

Immunoprecipitation:GGA1 AntibodyNBP3-30197] -Detection of human GGA1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GGA1 antibody NBP3-30197 (lot NBP3-30197-1) used for IP at 6 µg per reaction. GGA1 was also immunoprecipitated by rabbit anti-GGA1 antibody for blotting immunoprecipitated GGA1, NBP3-30197 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

GGA1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit GGA1 Antibody - BSA Free (NBP3-30197) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Between 425 and 475
• Assay Type: [Assay Type]
• Sample Volume: [Sample Volume]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GGA1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-citrate/phosphate pH 7 -8.
• Preservative: 0.09% Sodium Azide
• Purity: Affinity purified

Alternate Names for GGA1 Antibody - BSA Free

• ADP-ribosylation factor binding protein 1
• ADP-ribosylation factor-binding protein GGA1
• gamma adaptin ear containing, ARF binding protein 1
• gamma-adaptin related protein 1
• Gamma-adaptin-related protein 1
• golgi-associated, gamma adaptin ear containing, ARF binding protein 1
• Golgi-localized, gamma ear-containing, ARF-binding protein 1

Background

GGA1 encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) protein family. Members of this family are ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GGA1