Novus Biologicals products are now on bio-techne.com

GEM Antibody

Images

 
Western Blot: GEM Antibody [NBP2-26121] - HEK293 lysate overexpressing full-length Human GEM (HA tagged), mock-transfected HEK293 (EV) and HEK293 transiently expressing GEM-related genes (Rem, Rem2L and Rad) probed with ...read more
Western Blot: GEM Antibody [NBP2-26121] - (1ug/ml) staining of Human Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

GEM Antibody Summary

Immunogen
Peptide with sequence C-QKEPHQYSHRNRH corresponding to internal region according to NP_005252.1.
Specificity
Reported variants represent identical protein: NP_005252.1, NP_859053.1
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GEM
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 1 - 3 ug/ml
Application Notes
WB: In transfected HEK293 transiently expressing human recombinant GEM bands of approx. 35+45 kDa band are observed. These bands are not observed in the transfected HEK293 with empty vector and the antibody does not cross-react to related protein such as Rem, Rem2L or Rad, provided by Prof. R Andres, Univ. Kentucky, USA. A similar banding pattern is observed in human spleen. The calculated molecular size is 33.9 kDa band according to NP_005252.1.
Publications
Read Publication using NBP2-26121.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GEM Antibody

  • GTP binding protein overexpressed in skeletal muscle
  • GTP-binding mitogen-induced T-cell protein
  • GTP-binding protein GEM
  • GTP-binding protein overexpressed in skeletal muscle
  • kinase-inducible Ras-like protein
  • KIRMGC26294
  • RAS-like protein KIR

Background

The protein encoded by the GEM gene belongs to the RAD/GEM family of GTP-binding proteins. It is associated with the innerface of the plasma membrane and could play a role as a regulatory protein in receptor-mediated signal transduction.Alternative splicing occurs at this locus and two transcript variants encoding the same protein have been identified.(provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-61871
Species: Hu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
NBP2-50419
Species: Hu
Applications: CyTOF-ready, Flow, IP
MAB1058
Species: Hu
Applications: Block, CyTOF-ready, Flow
MAB2014
Species: Hu
Applications: CyTOF-reported, Flow
NB100-56618
Species: Hu, Mu
Applications: Flow-CS, Flow, ICC/IF, IHC, IHC-P, WB
MAB1059
Species: Hu
Applications: CyTOF-ready, Flow
H00003126-P01
Species: Hu
Applications: ELISA, AP, PA, WB
MAB1225
Species: Hu
Applications: Block, CyTOF-reported, Flow
MAB1844
Species: Hu
Applications: CyTOF-reported, Flow
MAB8887
Species: Hu
Applications: Flow
7268-CT
Species: Hu
Applications: BA
MAB2238
Species: Hu
Applications: AgAct, CyTOF-reported, Flow
NBP1-82874
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P
NBP2-79843
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
NB100-524
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
3015-KR
Species: Hu
Applications: BA
NBP2-36776
Species: Hu
Applications: ICC/IF, IHC, IHC-P, KD, WB
NBP2-12900
Species: Hu, Pm, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, MiAr, Simple Western, WB
NBP2-27500
Species: Mu
Applications: KO, PEP-ELISA, WB
NB120-6405
Species: Rt
Applications: B/N, CyTOF-ready, EM, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NBP1-58906
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
NBP2-26121
Species: Hu
Applications: WB, ELISA

Publications for GEM Antibody (NBP2-26121)(1)


Reviews for GEM Antibody (NBP2-26121) (0)

There are no reviews for GEM Antibody (NBP2-26121). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for GEM Antibody (NBP2-26121) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional GEM Products

Research Areas for GEM Antibody (NBP2-26121)

Find related products by research area.

Blogs on GEM

There are no specific blogs for GEM, but you can read our latest blog posts.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our GEM Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol GEM
Entrez