Mouse Gelsolin/GSN ELISA Kit (Colorimetric)

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 78.1 - 5000 ng/mL (example only; lot dependent)
• Sensitivity: 34 ng/mL (example only; lot dependent)

Mouse Gelsolin/GSN ELISA Kit (Colorimetric) Summary

• Description: Assay Length: 4.5 hours
• Standard Curve Range: 78.1 - 5000 ng/mL (example only; lot dependent)
• Sensitivity: 34 ng/mL (example only; lot dependent)
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 12 (example only; lot dependent)
• Intra-Assay: %CV < 10 (example only; lot dependent)
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: GSN

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse Gelsolin/GSN ELISA Kit (Colorimetric)

• Actin-depolymerizing factor
• ADF
• AGEL
• Brevin
• DKFZp313L0718
• gelsolin (amyloidosis, Finnish type)
• Gelsolin
• GSN

Background

Gelsolin is a calcium regulated actin binding protein. Gelsolin is a regulator of actin filament assembly and disassembly and consists of six homologous subdomains (S1-S6). When C-terminal tail (S6) is bound to calcium ion, it straightens and exposes actin binding sites located on S2s helices, leading to Gelsolin actin-serving activity (1). Gelsolin is inhibited by phosphatidylinositol (4,5)-bisphosphate (PIP2), where PIP2 will bind to S1, S2, and S3 sites. Gelsolin is involved in podosome formation and has been linked to inhibition of apoptosis by preventing release of cytochrome C (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Gelsolin/GSN ELISA Kit (NBP3-54392). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: GSN