Gelsolin/GSN Antibody

Western Blot: Gelsolin/GSN Antibody [NBP2-27566] - (1ug/ml) staining of Human Duodenum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Gelsolin/GSN Antibody Summary

• Description: Novus Biologicals Goat Gelsolin/GSN Antibody (NBP2-27566) is a polyclonal antibody validated for use in WB and ELISA. Anti-Gelsolin/GSN Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence PQGRVPEARPNS-C corresponding to N-Terminus according to NP_000168.1, NP_937895.1, NP_001121138.1.
• Specificity: This antibody is expected to recognize isoform a (NP_000168.1) only . Reported variants represent identical protein: NP_001121138.1, NP_001121139.1 Reported variants represent identical protein: NP_001121135.1, NP_937895.1, NP_001121134.1, NP_001121137.1, NP_001121136.1
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GSN
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 100 kDa band observed in human colon, duodenum, heart and skeletal muscle lysates (calculated MW of 85.7 kDa band according to NP_000168.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Gelsolin/GSN Antibody

• Actin-depolymerizing factor
• ADF
• AGEL
• Brevin
• DKFZp313L0718
• gelsolin (amyloidosis, Finnish type)
• Gelsolin
• GSN

Background

Gelsolin is a calcium regulated actin binding protein. Gelsolin is a regulator of actin filament assembly and disassembly and consists of six homologous subdomains (S1-S6). When C-terminal tail (S6) is bound to calcium ion, it straightens and exposes actin binding sites located on S2s helices, leading to Gelsolin actin-serving activity (1). Gelsolin is inhibited by phosphatidylinositol (4,5)-bisphosphate (PIP2), where PIP2 will bind to S1, S2, and S3 sites. Gelsolin is involved in podosome formation and has been linked to inhibition of apoptosis by preventing release of cytochrome C (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Gelsolin/GSN Antibody (NBP2-27566)(2)

Publications using NBP2-27566

• Shao Z, Lee X, Huang G et al. LINGO-1 Regulates Oligodendrocyte Differentiation through the Cytoplasmic Gelsolin Signaling Pathway. J. Neurosci. 2017-02-13 [PMID: 28193690] (WB, Human)
• An JH, Kim JW, Jang SM et al. Gelsolin negatively regulates the activity of tumor suppressor p53 through their physical interaction in hepatocarcinoma HepG2 cells. Biochem Biophys Res Commun 2011-08-19 [PMID: 21801713]

Bioinformatics

• Gene Symbol: GSN
• Uniprot:
  • NP_000168.1(Human)