Gelsolin/GSN Antibody (JB36-68)

Western Blot: Gelsolin/GSN Antibody (JB36-68) [NBP2-75503] - Analysis of Gelsolin on different lysates using anti-Gelsolin antibody at 1/500 dilution.Positive control:Lane 1: Mouse thymus Lane 2: Mouse lungLane 3: THP-1 Lane 4: HepG2

Immunocytochemistry/Immunofluorescence: Gelsolin/GSN Antibody (JB36-68) [NBP2-75503] - Staining Gelsolin in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Gelsolin/GSN Antibody (JB36-68) [NBP2-75503] - Analysis of paraffin-embedded mouse lung tissue using anti-Gelsolin antibody. Counter stained with hematoxylin.

Flow Cytometry: Gelsolin/GSN Antibody (JB36-68) [NBP2-75503] - Analysis of THP-1 cells with Gelsolin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: Gelsolin/GSN Antibody (JB36-68) [NBP2-75503] - Staining Gelsolin in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Gelsolin/GSN Antibody (JB36-68) [NBP2-75503] - Analysis of paraffin-embedded human kidney tissue using anti-Gelsolin antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Gelsolin/GSN Antibody (JB36-68) [NBP2-75503] - Analysis of paraffin-embedded human spleen tissue using anti-Gelsolin antibody. Counter stained with hematoxylin.

Product Discontinued

Gelsolin/GSN Antibody (JB36-68) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Gelsolin/GSN aa 431-480 / 782. (SwissProt: P06396 Human; SwissProt: P13020 Mouse)
• Localization: Cytoskeleton. Secreted.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: GSN
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 86 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Gelsolin/GSN Antibody (JB36-68)

• Actin-depolymerizing factor
• ADF
• AGEL
• Brevin
• DKFZp313L0718
• gelsolin (amyloidosis, Finnish type)
• Gelsolin
• GSN

Background

Gelsolin is a calcium regulated actin binding protein. Gelsolin is a regulator of actin filament assembly and disassembly and consists of six homologous subdomains (S1-S6). When C-terminal tail (S6) is bound to calcium ion, it straightens and exposes actin binding sites located on S2s helices, leading to Gelsolin actin-serving activity (1). Gelsolin is inhibited by phosphatidylinositol (4,5)-bisphosphate (PIP2), where PIP2 will bind to S1, S2, and S3 sites. Gelsolin is involved in podosome formation and has been linked to inhibition of apoptosis by preventing release of cytochrome C (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GSN