GEFT Antibody

Images

 
Western Blot: GEFT Antibody [NBP2-16636] - Sample (30 ug of whole cell lysate) A: IMR32 10% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: GEFT Antibody [NBP2-16636] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:250 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

GEFT Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human GEFT. The exact sequence is proprietary.
Localization
Cytoplasm; myofibril; sarcomere
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ARHGEF25
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Mouse (86%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GEFT Antibody

  • GEFTRAC/CDC42 exchange factor
  • Guanine nucleotide exchange factor GEFT
  • p63RhoGEFrho guanine nucleotide exchange factor 25
  • Rac/Cdc42/Rho exchange factor GEFT
  • Rho guanine nucleotide exchange factor (GEF) 25
  • RhoA/RAC/CDC42 exchange factor
  • RhoA/Rac/Cdc42 guanine nucleotide exchange factor GEFT

Background

The Rho family of small GTPases act as molecular switches to control a wide range of cellular processes. Guanine nucleotide exchange factors (GEFs), like GEFT, activate Rho GTPases by accelerating GDP/GTP exchange (Souchet et al., 2002 [PubMed 11861769]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for GEFT Antibody (NBP2-16636)

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Calculators

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Bioinformatics

Gene Symbol ARHGEF25