GCNF/NR6A1 Antibody (4G8) - BSA Free

Western Blot: GCNF/NR6A1 Antibody (4G8) [NBP2-37467] - Western blot analysis using NR6A1 mouse mAb against K562 (1), NTERA-2 (2), HEK293 (3), HUVE-12 (4), and HeLa (5) cell lysate.

Immunocytochemistry/Immunofluorescence: GCNF/NR6A1 Antibody (4G8) [NBP2-37467] - Immunofluorescence analysis of HepG2 cells using NR6A1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.

Immunohistochemistry: GCNF/NR6A1 Antibody (4G8) [NBP2-37467] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using NR6A1 mouse mAb with DAB staining.

Flow Cytometry: GCNF/NR6A1 Antibody (4G8) [NBP2-37467] - Flow cytometric analysis of K562 cells using NR6A1 mouse mAb (green) and negative control (red).

Western Blot: GCNF/NR6A1 Antibody (4G8) [NBP2-37467] - Western blot analysis using NR6A1 mAb against human NR6A1 recombinant protein. (Expected MW is 32.4 kDa)

Western Blot: GCNF/NR6A1 Antibody (4G8) [NBP2-37467] - Western blot analysis using NR6A1 mAb against HEK293 (1) and NR6A1 (AA: 65-118)-hIgGFc transfected HEK293 (2) cell lysate.

Immunohistochemistry: GCNF/NR6A1 Antibody (4G8) [NBP2-37467] - Immunohistochemical analysis of paraffin-embedded cerebellum tissues using NR6A1 mouse mAb with DAB staining.

ELISA: GCNF/NR6A1 Antibody (4G8) [NBP2-37467] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: 4G8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

GCNF/NR6A1 Antibody (4G8) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human GCNF/NR6A1 (AA: 65-118) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NR6A1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/ Immunofluorescence 1:400
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Application Notes: This antibody is Cytof ready.
• Theoretical MW: 54.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for GCNF/NR6A1 Antibody (4G8) - BSA Free

• GCNF
• GCNF1
• GCNFNR61
• Germ cell nuclear factor
• hGCNF
• NR6A1
• nuclear receptor subfamily 6 group A member 1
• nuclear receptor subfamily 6, group A, member 1
• retinoic acid receptor-related testis-associated receptor
• Retinoid receptor-related testis-specific receptor
• RTR
• RTRhRTR

Background

GCNF encodes an orphan nuclear receptor which is a member of the nuclear hormone receptor family. Its expression pattern suggests that it may be involved in neurogenesis and germ cell development. The protein can homodimerize and bind DNA, but in vivo targets have not been identified. The gene expresses at least alternatively spliced transcript variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NR6A1