Recombinant Human GCLM His Protein

SDS-Page: Recombinant Human GCLM Protein [NBP1-98869] - 15% SDS-PAGE (3ug)

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human GCLM His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-274 of Human GCLM
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMGTDSR AAKALLARAR TLHLQTGNLL NWGRLRKKCP STHSEELHDC IQKTLNEWSS QINPDLVREF PDVLECTVSH AVEKINPDER EEMKVSAKLF IVESNSSSST RSAVDMACSV LGVAQLDSVI IASPPIEDGV NLSLEHLQPY WEELENLVQS KKIVAIGTSD LDKTQLEQLY QWAQVKPNSN QVNLASCCVM PPDLTAFAKQ FDIQLLTHND PKELLSEASF QEALQESIPD IQAHEWVPLW LLRYSVIVKS RGIIKSKGYI LQAKRRGS

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: GCLM
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 33.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 10% glycerol, 0.15 M NaCl
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human GCLM His Protein

• Gamma-ECS regulatory subunit
• Gamma-glutamylcysteine synthetase regulatory subunit
• GCS light chain
• GLCLRglutamate--cysteine ligase regulatory subunit
• glutamate-cysteine ligase (gamma-glutamylcysteine synthetase), regulatory(30.8kD)
• Glutamate--cysteine ligase modifier subunit
• glutamate-cysteine ligase regulatory protein
• glutamate-cysteine ligase, modifier subunit
• GSC light chain

Background

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. Recombinant human GCLM protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: GCLM