GCLM Antibody (JM93-61)

Western Blot: GCLM Antibody (JM93-61) [NBP2-75502] - Analysis of GCLM on different cell lysates using anti-GCLM antibody at 1/500 dilution.Positive control:Lane 1: A431 Lane 2: PC-12Lane 3: NIH-3T3

Immunocytochemistry/Immunofluorescence: GCLM Antibody (JM93-61) [NBP2-75502] - Staining GCLM in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: GCLM Antibody (JM93-61) [NBP2-75502] - Analysis of paraffin-embedded mouse small intestine tissue using anti-GCLM antibody. Counter stained with hematoxylin.

Flow Cytometry: GCLM Antibody (JM93-61) [NBP2-75502] - Analysis of Hela cells with GCLM antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

Immunocytochemistry/Immunofluorescence: GCLM Antibody (JM93-61) [NBP2-75502] - Staining GCLM in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: GCLM Antibody (JM93-61) [NBP2-75502] - Staining GCLM in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: GCLM Antibody (JM93-61) [NBP2-75502] - Analysis of paraffin-embedded human colon cancer tissue using anti-GCLM antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: GCLM Antibody (JM93-61) [NBP2-75502] - Analysis of paraffin-embedded human pancreas tissue using anti-GCLM antibody. Counter stained with hematoxylin.

Product Discontinued

GCLM Antibody (JM93-61) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human GCLM aa 225-274 / 274. (SwissProt: P48507 Human; SwissProt: O09172 Mouse; SwissProt: P48508 Rat)
• Localization: Cytosol.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: GCLM
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Immunoprecipitation
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for GCLM Antibody (JM93-61)

• Gamma-ECS regulatory subunit
• Gamma-glutamylcysteine synthetase regulatory subunit
• GCS light chain
• GLCLRglutamate--cysteine ligase regulatory subunit
• glutamate-cysteine ligase (gamma-glutamylcysteine synthetase), regulatory(30.8kD)
• Glutamate--cysteine ligase modifier subunit
• glutamate-cysteine ligase regulatory protein
• glutamate-cysteine ligase, modifier subunit
• GSC light chain

Background

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GCLM