GCH1 Antibody

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Western Blot: GCH1 Antibody [NBP1-20923] - (0.5ug/ml) staining of Human Tonsil lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

GCH1 Antibody Summary

Immunogen
Peptide with sequence C-GKVHIGYLPNKQ corresponding to internal region according to NP_001019195.1, NP_001019241.1, NP_001019242.1.
Epitope
C terminus
Specificity
This antibody is expected to recognize all four isoforms (NP_000152.1 ; NP_001019195.1 ; NP_001019241.1 ; NP_001019242.1)
Predicted Species
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GCH1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 - 1.5 ug/ml
  • Peptide ELISA Detection limit 1:16000
Application Notes
WB: Approx. 37 kDa band observed in human tonsil, lymph node and thymus lysates. An anonymous customer has reported identical results in HUVEC lysates with antibodies raised against different parts of the same protein (calculated MW of 27.9 kDa band according to NP_000152.1).
Publications
Read Publication using NBP1-20923.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GCH1 Antibody

  • dystonia 14
  • DYT14
  • DYT5
  • DYT5a
  • DYT5EC 3.5.4.16
  • GCH
  • GCH1
  • GTP cyclohydrolase 1
  • GTP cyclohydrolase I
  • GTPCH1
  • GTP-CH-1
  • GTP-CH-I
  • guanosine 5'-triphosphate cyclohydrolase I
  • HPABH4B

Background

This gene encodes a member of the GTP cyclohydrolase family. The encoded protein is the first and rate-limiting enzyme in tetrahydrobiopterin (BH4) biosynthesis, catalyzing the conversion of GTP into 7,8-dihydroneopterin triphosphate. BH4 is an essential cofactor required by aromatic amino acid hydroxylases as well as nitric oxide synthases. Mutations in this gene are associated with malignant hyperphenylalaninemia and dopa-responsive dystonia. Several alternatively spliced transcript variants encoding different isoforms have been described; however, not all variants give rise to a functional enzyme.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GCH1 Antibody (NBP1-20923)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Bioinformatics

Gene Symbol GCH1
Entrez
Uniprot