GBL Antibody

Product Discontinued

GBL Antibody Summary

• Immunogen: Antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human GbL. The immunogen is located within the first 50 amino acids of GBL.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MLST8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: GbL antibody can be used for the detection of GbL by Western blot at 1 mg/ml.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GBL Antibody

• G protein beta subunit-like
• gable
• GbetaL
• GBL
• GBLPop3
• Lst8
• LST8MGC111011
• Mammalian lethal with SEC13 protein 8
• mLST8
• MTOR associated protein, LST8 homolog (S. cerevisiae)
• POP3
• Protein GbetaL
• target of rapamycin complex subunit LST8
• TORC subunit LST8
• WAT1

Background

GbetaL (G protein beta protein subunit-like) is a member of a signaling pathway that regulates mammalian cell growth in response to the presence of nutrients and growth factors. It binds to the kinase domain of TOR (Target of rapamycin, also known as mTOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. Rapamycin inhibits TOR resulting in reduced cell growth and reduced rates of cell cycle and cell proliferation. TOR is normally associated with GbetaL and an additional regulatory protein RAPTOR, allowing TOR to control protein biosynthesis. The binding of GbetaL to TOR stimulates TOR's kinase activity towards downstream proteins such as RPS6K (ribosomal protein S6 kinase) and the translation factor 4E-BP1 which leads to increased protein translation and cell growth.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MLST8
• Uniprot:
  • AAH52292(Human)