Gankyrin Antibody (4B5)

Product Discontinued

Gankyrin Antibody (4B5) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: PSMD10 (AAH11960 127 a.a. - 226 a.a.) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EGGANPDAKDHYEATAMHRAAAKGNLKMIHILLYYKASTNIQDTEGNTPLHLACDEERVEEAKLLVSQGASIYIENKEEKTPLQVAKGGLGLILKRMVEG
• Specificity: PSMD10 - proteasome (prosome, macropain) 26S subunit, non-ATPase, 10
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PSMD10
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Gankyrin Antibody (4B5)

• 26S proteasome non-ATPase regulatory subunit 10
• 26S proteasome regulatory subunit p28
• ankyrin repeat protein
• dJ889N15.2
• gankyrin
• hepatocellular carcinoma-associated protein p28-II
• p28
• p28(GANK)
• proteasome (prosome, macropain) 26S subunit, non-ATPase, 10

Background

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. Two transcripts encoding different isoforms have been described. Pseudogenes have been identified on chromosomes 3 and 20.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PSMD10
• Entrez:
  • 5716(Human)
• OMIM:
  • 603480(Human)
• Uniprot:
  • AAH11960(Human)