Immunohistochemistry: gamma H2AX [p Ser139] Antibody [NB100-2280] - Biological and molecular analysis of cutaneous wound healing in p73+/+ and p73-/- mice. Representative micrographs of immunohistochemistry (IHC) ...read more
Simple Western: gamma H2AX [p Ser139] Antibody [NB100-2280] - Simple Western lane view shows a specific band for gamma H2AX in 0.2 mg/ml of Jurkat lysate(s). This experiment was performed under reducing conditions ...read more
Immunohistochemistry: gamma H2AX [p Ser139] Antibody [NB100-2280] - Telomere dysfunctional induced foci (TIF) in HBV-related multistep hepatocarcinogenesis and the correlations thereof with stathmin and elongation ...read more
Immunohistochemistry-Paraffin: gamma H2AX [p Ser139] Antibody [NB100-2280] - Detection of human gamma H2AX [p Ser139] Antibody by immunohistochemistry. Sample: FFPE section of human seminoma. Antibodies: Affinity ...read more
Immunohistochemistry: gamma H2AX [p Ser139] Antibody [NB100-2280] - Detection of Mouse gamma-H2AX by Immunohistochemistry. Sample: FFPE section of mouse colon carcinoma CT26. Antibodies: Affinity purified rabbit ...read more
Simple Western: gamma H2AX [p Ser139] Antibody [NB100-2280] - Electropherogram image(s) of corresponding Simple Western lane view. Gamma H2AX antibody was used at 1:20 dilution on Jurkat lysate(s).
Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining. Likely to work with frozen sections. Use in WB reported in scientific literature ( PMID 24415760). Use in IHC-Frozen reported in scientific literature (PMID 26577699).
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Canine reactivity reported in scientific literature (PMID: 26991424).
Packaging, Storage & Formulations
Store at 4C. Do not freeze.
TBS and 0.1% BSA
0.09% Sodium Azide
Immunogen affinity purified
Licensed to Novus Biologicals LLC under U.S. Patent Nos. 6,362,317 and 6,884,873.
Alternate Names for gamma H2AX [p Ser139] Antibody
H2A histone family, member X
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. H2AX is an early responder to DNA damage, and its change to what is known as gamma-H2AX is important for the coordination of signaling and repair activities.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for gamma H2AX Antibody (NB100-2280). (Showing 1 - 1 of 1 FAQs).
I am interested to order a pair of antibodies (rabbit) for H2AX/ foci analysis. I have selected these two (catalog # NB100-2280 and NB100-79967) that could be suitable for my experiment. However, I have a difficulty to select the appropriate secondary antibody. Do you have any suggestion for an appropriate secondary rabbit antibody or an appropriate package of pairs of rabbit antibodies suitable for H2AX?
What application are you trying to do? What would you like to have the secondary conjugated to? Unfortunately, I am not able to recommend a secondary antibody for you until I know more detail about what you intend to do with the primaries. Here is a link to our anti-rabbit secondary antibodies can be found at the following link.
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Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.