gamma H2AX [p Ser139] Antibody

Images

 
Immunohistochemistry: gamma H2AX [p Ser139] Antibody [NB100-2280] - Biological and molecular analysis of cutaneous wound healing in p73+/+ and p73-/- mice. Representative micrographs of immunohistochemistry (IHC) ...read more
Simple Western: gamma H2AX [p Ser139] Antibody [NB100-2280] - Simple Western lane view shows a specific band for gamma H2AX in 0.2 mg/ml of Jurkat lysate(s). This experiment was performed under reducing conditions ...read more
Immunohistochemistry: gamma H2AX [p Ser139] Antibody [NB100-2280] - Telomere dysfunctional induced foci (TIF) in HBV-related multistep hepatocarcinogenesis and the correlations thereof with stathmin and elongation ...read more
Immunohistochemistry-Paraffin: gamma H2AX [p Ser139] Antibody [NB100-2280] - Detection of human gamma H2AX [p Ser139] Antibody by immunohistochemistry. Sample: FFPE section of human seminoma. Antibodies: Affinity ...read more
Immunohistochemistry: gamma H2AX [p Ser139] Antibody [NB100-2280] - Detection of Mouse gamma-H2AX by Immunohistochemistry. Sample: FFPE section of mouse colon carcinoma CT26. Antibodies: Affinity purified rabbit ...read more
Simple Western: gamma H2AX [p Ser139] Antibody [NB100-2280] - Electropherogram image(s) of corresponding Simple Western lane view. Gamma H2AX antibody was used at 1:20 dilution on Jurkat lysate(s).

Product Details

Summary
Reactivity Hu, Mu, CaSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

gamma H2AX [p Ser139] Antibody Summary

Immunogen
Immunogen was a synthetic phospho-peptide, which represented a portion of the C-terminus of human histone H2AX surrounding phosphorylated serine 139 (GeneID 3014).
Modification
p Ser139
Marker
DNA Double-strand break marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
H2AX
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Simple Western 1:20
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:500
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100-1:500
Application Notes
Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining. Likely to work with frozen sections. Use in WB reported in scientific literature ( PMID 24415760). Use in IHC-Frozen reported in scientific literature (PMID 26577699).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Control
Histone H2AX Overexpression Lysate
Reviewed Applications
Read 1 Review rated 5
using
NB100-2280 in the following applications:

Publications
Read Publications using
NB100-2280 in the following applications:

Reactivity Notes

Canine reactivity reported in scientific literature (PMID: 26991424).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Purity
Immunogen affinity purified

Notes

Licensed to Novus Biologicals LLC under U.S. Patent Nos. 6,362,317 and 6,884,873.

Alternate Names for gamma H2AX [p Ser139] Antibody

  • H2A histone family, member X
  • H2A.X
  • H2a/x
  • H2AX histone
  • H2AXhistone H2A.x
  • YH2AX

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. H2AX is an early responder to DNA damage, and its change to what is known as gamma-H2AX is important for the coordination of signaling and repair activities.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for gamma H2AX Antibody (NB100-2280)(22)

We have publications tested in 3 confirmed species: Human, Mouse, Canine.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
ICC/IF
(6)
IHC
(1)
IHC-Fr
(1)
IHC-P
(6)
WB
(4)
All Applications
Filter By Species
Human
(8)
Mouse
(7)
Canine
(1)
All Species
Showing Publications 1 - 10 of 22. Show All 22 Publications.
Publications using NB100-2280 Applications Species
Singh AN, Oehler J, Torrecilla I et al. The p97-Ataxin 3 complex regulates homeostasis of the DNA damage response E3 ubiquitin ligase RNF8 EMBO J. 2019 Oct 15 [PMID: 31613024] (WB, Human) WB Human
Beeler JS, Marshall CB, Gonzalez-Ericsson PI, et al. p73 regulates epidermal wound healing and induced keratinocyte programming PLoS ONE 2019 Jun 19 [PMID: 31216312] (IHC-P, Mouse) IHC-P Mouse
Cespedes MV, Guillen MJ, Lopez-Casas PP et al. Lurbinectedin induces depletion of tumor-associated macrophages, an essential component of its in vivo synergism with gemcitabine, in pancreatic adenocarcinoma mouse models. Dis Model Mech. Dec 1 2016 [PMID: 27780828] (IHC, Human) IHC Human
Lopez KA Therapeutic targeting of histone H3 lysine 36 trimethylation-deficient cancers Thesis 2019 (ICC/IF, Human) ICC/IF Human
Ma H, Yang F, Ding XQ Inhibition of thyroid hormone signaling protects retinal pigment epithelium and photoreceptors from cell death in a mouse model of age-related macular degeneration Cell Death Dis Jan 13 2020 [PMID: 31932580] (ICC/IF, Mouse) ICC/IF Mouse
Lu M, Hartmann D, Braren R et al. Oncogenic Akt-FOXO3 loop favors tumor-promoting modes and enhances oxidative damage-associated hepatocellular carcinogenesis BMC Cancer Sep 5 2019 [PMID: 31488102] (IHC-P, Mouse) IHC-P Mouse
Burdova K, Yang H, Faedda R et al. E2F1 proteolysis via SCF-cyclin F underlies synthetic lethality between cyclin F loss and Chk1 inhibition EMBO J. Aug 19 2019 [PMID: 31424118] (WB, Human) WB Human
Beeler JSA The Role of p73 in Basal Keratinocyte Function Thesis (ICC/IF, Mouse) ICC/IF Mouse
Wen J, Yan H, He M et al. GSK-3b protects fetal oocytes from premature death via modulating TAp63 expression in mice BMC Biol. Mar 12 2019 [PMID: 30866939] (ICC/IF, Mouse) ICC/IF Mouse
Jamal S, Cheriyan VT, Muthu M et al. Establishment and characterization of a canine soft tissue sarcoma patient-derived xenograft model. PLOS One [PMID: 26991424] (IHC-P, Canine) IHC-P Canine
Show All 22 Publications.

Review for gamma H2AX Antibody (NB100-2280) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-2280:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin gamma H2AX NB100-2280
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reviewed by:
Anonymous
IHC-P Human 10/06/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedRamos lymphoma
SpeciesHuman
Lot4

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for gamma H2AX Antibody (NB100-2280). (Showing 1 - 1 of 1 FAQs).

  1. I am interested to order a pair of antibodies (rabbit) for H2AX/ foci analysis. I have selected these two (catalog # NB100-2280 and NB100-79967) that could be suitable for my experiment. However, I have a difficulty to select the appropriate secondary antibody. Do you have any suggestion for an appropriate secondary rabbit antibody or an appropriate package of pairs of rabbit antibodies suitable for H2AX?
    • What application are you trying to do? What would you like to have the secondary conjugated to? Unfortunately, I am not able to recommend a secondary antibody for you until I know more detail about what you intend to do with the primaries. Here is a link to our anti-rabbit secondary antibodies can be found at the following link.

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Blogs on gamma H2AX.

Further unraveling the role of gamma H2AX in DNA damage response
Our genome experiences a moderate amount of DNA damage in our cells on a daily basis.  This DNA damage can be in response to external environmental factors, or be a result of our internal metabolic processes going awry.  While normal rates of DNA ...  Read full blog post.

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Recent Reviews

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Anonymous
10/06/2015
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol H2AX
Entrez
Uniprot