gamma H2AX [p Ser139] Antibody (3F2)

Images

 
Simple Western: gamma H2AX [p Ser139] Antibody (3F2) [NB100-74435] - Simple Western lane view shows a specific band for gamma H2AX in 0.2 mg/ml of Jurkat lysate(s). This experiment was performed under reducing ...read more
Biological Strategies: Immunocytochemistry/ Immunofluorescence: gamma H2AX [p Ser139] Antibody (3F2) [NB100-74435] - Staining of Phospho-H2AX in A549 cells. Cells were treated with 50 uM EPE for 1 hour (left ...read more
Immunocytochemistry/ Immunofluorescence: gamma H2AX [p Ser139] Antibody (3F2) [NB100-74435] - Staining using NB100-74435, treatment with paraquat and iron induces MnSOD and Phosphorylation of H2AX in RAW 264.7 ...read more
Biological Strategies: Western Blot: gamma H2AX [p Ser139] Antibody (3F2) [NB100-74435] - Analysis of gamma H2AX antibody.
Immunocytochemistry/ Immunofluorescence: gamma H2AX [p Ser139] Antibody (3F2) [NB100-74435] - Evidence of DNA damage and oxidative stress in islets from HFD treated mice. Representative immunofluorescent staining of ...read more
Western Blot: gamma H2AX [p Ser139] Antibody (3F2) [NB100-74435] - Figure 1 illustrates WB analysis of phosphorylated H2AX in gamma irradiated Hela cell lysate. Lane 1: Unphosphorylated H2AX control Lane 2: ...read more
Immunocytochemistry/ Immunofluorescence: gamma H2AX [p Ser139] Antibody (3F2) [NB100-74435] - Analysis of Phospho-Histone H2A.X (Ser139) using a monoclonal antibody.
Simple Western: gamma H2AX [p Ser139] Antibody (3F2) [NB100-74435] - Electropherogram image(s) of corresponding Simple Western lane view. Gamma H2AX antibody was used at 10 ug/ml dilution on Jurkat lysate(s).

Product Details

Summary
Reactivity Hu, Mu, BvSpecies Glossary
Applications WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-P
Clone
3F2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

gamma H2AX [p Ser139] Antibody (3F2) Summary

Immunogen
Synthetic peptide sequence surrounding phosphorylated Ser139
Modification
p Ser139
Marker
DNA Double-strand break marker
Specificity
H2AX [Ser139] Phosphospecific (3F2) [Ser139]
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
H2AX
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • Simple Western 10 ug/ml
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1 ug/million cells
  • Immunocytochemistry/Immunofluorescence 2 - 4 ug/ml
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
Application Notes
In WB: Detects an approx. 17 kDa protein representing phosphorylated H2AX in gamma irradiate Hela cell lysate.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Control
Histone H2AX Overexpression Lysate
Publications
Read Publications using
NB100-74435 in the following applications:

  • IHC
    1 publication
  • 2 publications
  • WB
    2 publications

Reactivity Notes

Bovine reactivity reported in scientific literature (PMID: 17604361). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 1 mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Notes

Licensed to Novus Biologicals LLC under U.S. Patent Nos. 6,362,317 and 6,884,873.

Alternate Names for gamma H2AX [p Ser139] Antibody (3F2)

  • H2A histone family, member X
  • H2A.X
  • H2a/x
  • H2AX histone
  • H2AXhistone H2A.x
  • YH2AX

Background

Histones are basic nuclear proteins that are responsible for the structure of eukaryotic chromosomal fiber. H2AX is a member of the histone H2A family which is one of the four core histones making up the nucleosome core particle. In eukaryotes DNA double strand breaks (DSBs) have been shown to trigger the phosphorylation of serine 139 at the carboxy terminus of histone H2AX resulting in gamma-H2AX. The phosphorylation of H2AX can be detected by Western blotting or immunofluorescence, revealing the frequency of DSBs. The phosphatidylinositol 3-kinases have been implicated in H2AX phosphorylation, but it is unclear if ATM is the primary H2AX kinase or if other members of the family such as DNA-PK and ATR contribute in a similar manner.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for gamma H2AX Antibody (NB100-74435)(5)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for gamma H2AX Antibody (NB100-74435) (0)

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Control Lysate(s)

Secondary Antibodies

 

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Blogs on gamma H2AX.

Further unraveling the role of gamma H2AX in DNA damage response
Our genome experiences a moderate amount of DNA damage in our cells on a daily basis.  This DNA damage can be in response to external environmental factors, or be a result of our internal metabolic processes going awry.  While normal rates of DNA ...  Read full blog post.

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Bioinformatics

Gene Symbol H2AX
Entrez
Uniprot