Simple Western: gamma H2AX [p Ser139] Antibody (3F2) [NB100-74435] - Simple Western lane view shows a specific band for gamma H2AX in 0.2 mg/ml of Jurkat lysate(s). This experiment was performed under reducing ...read more
Biological Strategies: Immunocytochemistry/ Immunofluorescence: gamma H2AX [p Ser139] Antibody (3F2) [NB100-74435] - Staining of Phospho-H2AX in A549 cells. Cells were treated with 50 uM EPE for 1 hour (left ...read more
Immunocytochemistry/ Immunofluorescence: gamma H2AX [p Ser139] Antibody (3F2) [NB100-74435] - Staining using NB100-74435, treatment with paraquat and iron induces MnSOD and Phosphorylation of H2AX in RAW 264.7 ...read more
Biological Strategies: Western Blot: gamma H2AX [p Ser139] Antibody (3F2) [NB100-74435] - Analysis of gamma H2AX antibody.
Immunocytochemistry/ Immunofluorescence: gamma H2AX [p Ser139] Antibody (3F2) [NB100-74435] - Evidence of DNA damage and oxidative stress in islets from HFD treated mice. Representative immunofluorescent staining of ...read more
Western Blot: gamma H2AX [p Ser139] Antibody (3F2) [NB100-74435] - Figure 1 illustrates WB analysis of phosphorylated H2AX in gamma irradiated Hela cell lysate. Lane 1: Unphosphorylated H2AX control Lane 2: ...read more
Immunocytochemistry/ Immunofluorescence: gamma H2AX [p Ser139] Antibody (3F2) [NB100-74435] - Analysis of Phospho-Histone H2A.X (Ser139) using a monoclonal antibody.
Simple Western: gamma H2AX [p Ser139] Antibody (3F2) [NB100-74435] - Electropherogram image(s) of corresponding Simple Western lane view. Gamma H2AX antibody was used at 10 ug/ml dilution on Jurkat lysate(s).
Bovine reactivity reported in scientific literature (PMID: 17604361). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Packaging, Storage & Formulations
Store at -20C. Avoid freeze-thaw cycles.
PBS with 1 mg/ml BSA
0.05% Sodium Azide
Protein G purified
Licensed to Novus Biologicals LLC under U.S. Patent Nos. 6,362,317 and 6,884,873.
Alternate Names for gamma H2AX [p Ser139] Antibody (3F2)
H2A histone family, member X
Histones are basic nuclear proteins that are responsible for the structure of eukaryotic chromosomal fiber. H2AX is a member of the histone H2A family which is one of the four core histones making up the nucleosome core particle. In eukaryotes DNA double strand breaks (DSBs) have been shown to trigger the phosphorylation of serine 139 at the carboxy terminus of histone H2AX resulting in gamma-H2AX. The phosphorylation of H2AX can be detected by Western blotting or immunofluorescence, revealing the frequency of DSBs. The phosphatidylinositol 3-kinases have been implicated in H2AX phosphorylation, but it is unclear if ATM is the primary H2AX kinase or if other members of the family such as DNA-PK and ATR contribute in a similar manner.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.