gamma Catenin Antibody

Images

 
Western Blot: gamma Catenin Antibody [NB100-1060] - Staining (1ug/ml) of Rat Eye lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry-Paraffin: gamma Catenin Antibody [NB100-1060] - staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: gamma Catenin Antibody [NB100-1060] - Staining of A431 (A) and NIH3T3 (B) cell lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

gamma Catenin Antibody Summary

Immunogen
Peptide with sequence C-GLRPPYPTADHMLA corresponding to C-Terminus according to NP_002221.1, NP_068831.1.
Localization
Cytoplasmic in a soluble and membrane-associated form.
Specificity
This antibody is expected to recognise both reported isoforms, as represented by NP_002221.1 and NP_068831.1.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
JUP
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 0.1 - 0.3 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
EIA: An anonymous customer has reported successful usage quantitative ELISA assays (EIA), very sensitive to freeze/thaw cycles. WB: Approx. 85 kDa band observed in lysates of A431A 549 and NIH3T3 and in rat eye lysates (calculated MW of 81.7 kDa band according to human NP_002221.1 and mouse NP_034723.1).
IHC-Paraffin embedded Human Heart.
Positive Control
A-431 Lysate (NB820-59461)
gamma Catenin Lysate (NBL1-12118)
Publications
Read Publication using NB100-1060.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Canine, Porcine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for gamma Catenin Antibody

  • catenin (cadherin-associated protein), gamma (80kD)
  • catenin (cadherin-associated protein), gamma 80kDa
  • Catenin gamma
  • Desmoplakin III
  • desmoplakin-3
  • DP3CTNNG
  • DPIII
  • gamma-catenin
  • junction plakoglobin
  • PDGBARVD12
  • PKGB

Background

Plakoglobin is a major cytoplasmic protein which is the only known constituent common to submembranous plaques of both desmosomes and intermediate junctions. This protein forms distinct complexes with cadherins and desmosomal cadherins and is a member of the catenin family since it contains a distinct repeating amino acid motif called the armadillo repeat. Mutation in this gene has been associated with Naxos disease. Alternative splicing occurs in this gene; however, not all transcripts have been fully described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for gamma Catenin Antibody (NB100-1060)(1)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on gamma Catenin.

Beta Catenin in Cell Adhesion and T-cell Signaling
Beta Catenin is a cytosolic, 88 kDa intracellular protein that tightly associates with cell surface cadherin glycoproteins. It is one member of the catenin family that includes alpha Catenin, beta Catenin, and gamma Catenin. Colocalization studies usi...  Read full blog post.

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Bioinformatics

Gene Symbol JUP
Entrez
Uniprot