Human Galectin-3 ELISA Kit (Colorimetric)

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ELISA: Galectin-3 ELISA Kit [NBP1-91275] - Standard curve

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell culture supernatant, serum, plasma (EDTA)
Standard Curve Range
0.39 - 25.0 ng/mL
Sensitivity
0.12 ng/mL

Order Details

Human Galectin-3 ELISA Kit (Colorimetric) Summary

Standard Curve Range
0.39 - 25.0 ng/mL
Sensitivity
0.12 ng/mL
Assay Type
Sandwich ELISA
Inter-Assay
5.40%
Intra-Assay
7.50%
Spike Recovery
Please See Protocol for Specific Spike Recovery Details
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
LGALS3

Applications/Dilutions

Application Notes
The human Galectin-3 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human Galectin-3
Publications
Read Publications using
NBP1-91275 in the following applications:

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Stop Solution (1M Phosphoric acid)
  2. Sample Diluent
  3. Substrate Solution (tetramethyl-benzidine)
  4. Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
  5. Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human Galectin-3
  6. Adhesive Films
  7. Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  8. Human Galectin-3 Standard lyophilized, 60 ng/ml upon reconstitution
  9. HRP-Conjugate anti-human Galectin-3 monoclonal antibody

Alternate Names for Human Galectin-3 ELISA Kit (Colorimetric)

  • AGE-R3
  • Carbohydrate-binding protein 35
  • CBP 35,35 kDa lectin
  • CBP35
  • GAL3
  • Gal-3
  • galactin-3
  • Galactose-specific lectin 3
  • Galactoside-binding protein
  • GALBPCBP35
  • Galectin3
  • Galectin-3
  • GALIG
  • IgE-binding protein
  • L29
  • L31
  • L-31
  • Laminin-binding protein
  • Lectin L-29
  • lectin, galactoside-binding, soluble, 3
  • LGALS2
  • LGALS3
  • Mac-2 antigen
  • Mac-2
  • MAC2GAL3

Background

The human Galectin-3 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human Galectin-3. Galectin-3 is a 26kDa -galactoside-binding protein belonging to the Galectin family, which consists of more than ten members. Galectin-3 is composed of a carboxyl-terminal carbohydrate recognition domain (CRD) and amino-terminal tandem repeats. Galectin-3 normally distributes in epithelia of many organs and various inflammatory cells, including macrophages as well as dendritic cells and Kupfer cells. The expression of this lectin is up-regulated during inflammation, cell proliferation and cell differentiation and through transactivation by viral proteins. Galectin-3 has been shown to function through both intracellular and extracellular actions. It is a component of heterogeneous nuclear ribonuclear protein (hnRNP), a factor in pre-mRNA splicing and has been found to control cell cycle and prevent T-cell apoptosis through interaction with the Bcl-2 family members. On the other hand, this protein, which is secreted from monocytes/macrophages and epithelial cells has been demonstrated to function as an extracellular molecule in activating various types of cells such as monocytes/macrophages, mast cells, neutrophils and lymphocytes. Galectin-3 has been shown to mediate cell-cell and cell-extracellular matrix interactions and acts as a novel chemoattractant for monocytes and macrophages.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Publications for Galectin-3 ELISA Kit (NBP1-91275)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ELISA.


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Bioinformatics

Gene Symbol LGALS3
Entrez