Human Galectin-3 ELISA Kit (Colorimetric)

ELISA: Galectin-3 ELISA Kit [NBP1-91275] - Standard curve

Product Discontinued

Human Galectin-3 ELISA Kit (Colorimetric) Summary

• Description: The human Galectin-3 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human Galectin-3
• Specificity: The assay detects both natural and recombinant Galectin-3. The crossreactivity and interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a Galectin-3 positive sample. No cross-reactivity or interference was detected.
• Standard Curve Range: 0.39 - 25.0 ng/mL
• Sensitivity: 0.12 ng/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 5.40%
• Intra-Assay: CV% < 7.50%
• Spike Recovery: Please See Protocol for Specific Spike Recovery Details
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: LGALS3

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Galectin-3 ELISA Kit (Colorimetric)

• AGE-R3
• Carbohydrate-binding protein 35
• CBP 35,35 kDa lectin
• CBP35
• GAL3
• Gal-3
• galactin-3
• Galactose-specific lectin 3
• Galactoside-binding protein
• GALBPCBP35
• Galectin3
• Galectin-3
• GALIG
• IgE-binding protein
• L29
• L31
• L-31
• Laminin-binding protein
• Lectin L-29
• lectin, galactoside-binding, soluble, 3
• LGALS2
• LGALS3
• Mac-2 antigen
• Mac-2
• MAC2GAL3

Background

The human Galectin-3 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human Galectin-3. Galectin-3 is a 26kDa -galactoside-binding protein belonging to the Galectin family, which consists of more than ten members. Galectin-3 is composed of a carboxyl-terminal carbohydrate recognition domain (CRD) and amino-terminal tandem repeats. Galectin-3 normally distributes in epithelia of many organs and various inflammatory cells, including macrophages as well as dendritic cells and Kupfer cells. The expression of this lectin is up-regulated during inflammation, cell proliferation and cell differentiation and through transactivation by viral proteins. Galectin-3 has been shown to function through both intracellular and extracellular actions. It is a component of heterogeneous nuclear ribonuclear protein (hnRNP), a factor in pre-mRNA splicing and has been found to control cell cycle and prevent T-cell apoptosis through interaction with the Bcl-2 family members. On the other hand, this protein, which is secreted from monocytes/macrophages and epithelial cells has been demonstrated to function as an extracellular molecule in activating various types of cells such as monocytes/macrophages, mast cells, neutrophils and lymphocytes. Galectin-3 has been shown to mediate cell-cell and cell-extracellular matrix interactions and acts as a novel chemoattractant for monocytes and macrophages.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Galectin-3 ELISA Kit (NBP1-91275)(2)

Publications using NBP1-91275

• Lala RI, Lungeanu D, Darabantiu D et al. Galectin-3 as a marker for clinical prognosis and cardiac remodeling in acute heart failure. Herz. 2017-02-24 [PMID: 28235980]
• Lala RI, Darabantiu D, Pilat L et al. Galectin-3: A Link between Myocardial and Arterial Stiffening in Patients with Acute Decompensated Heart Failure. Arq. Bras. Cardiol. 2016-01-12 [PMID: 26760784] (ELISA, Human)

FAQs for Galectin-3 ELISA Kit (NBP1-91275). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: LGALS3