Recombinant Human Galectin-3 Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
Lyoph

Order Details

Recombinant Human Galectin-3 Protein Summary

Description
Recombinant bioactive protein containing 250 amino acids for Human Galectin-3

Source:E. coli
Amino Acid Sequence:(P17931)

Preparation
Method
Determined to be >98% pure by SDS-PAGE
Details of Functionality
Determined by its ability to chemoattract human blood monocytes. Chemotactic activity was observed at a concentration of 2.5 ug/ml with a peak response obtained at 250 ug/ml.
Source
E. coli
Protein/Peptide Type
Biologically Active Protein
Gene
LGALS3
Purity
>95%, by SDS-PAGE
Endotoxin Note
<0.1 ng/ug

Applications/Dilutions

Application Notes
Unit Definition: One unit is defined as the amount of enzyme that hydrolyzes 1 nmol/min of Ac-IETD-PNA per min at 25oC in 50 mM Tris pH 8.0 containing 150 mM NaCl and 0.01% Triton X-100.
Theoretical MW
26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
Sterile filtered and lyophilized from a concentrated solution containing 0.5X PBS, pH 7.2.
Concentration
Lyoph
Purity
>95%, by SDS-PAGE
Reconstitution Instructions
Reconstitute in H2O to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4C for 1 week or -20C for future use.

Alternate Names for Recombinant Human Galectin-3 Protein

  • AGE-R3
  • Carbohydrate-binding protein 35
  • CBP 35,35 kDa lectin
  • CBP35
  • GAL3
  • Gal-3
  • galactin-3
  • Galactose-specific lectin 3
  • Galactoside-binding protein
  • GALBPCBP35
  • Galectin3
  • Galectin-3
  • GALIG
  • IgE-binding protein
  • L29
  • L31
  • L-31
  • Laminin-binding protein
  • Lectin L-29
  • lectin, galactoside-binding, soluble, 3
  • LGALS2
  • LGALS3
  • Mac-2 antigen
  • Mac-2
  • MAC2GAL3

Background

Lectins are carbohydrate binding proteins that interact with glycoprotein and glycolipids on the surface of animal cells. The Galectins are lectins that recognize and interact with Beta -galactoside moieties. Galactin-3 regulates a number of biological processes, including embryogenesis, inflammatory responses, cell progression and metastasis. Galectin-3 can function intraCellularly in controlling cell cycle and preventing T-cell apoptosis, and also extraCellularly, in activating various cells, including monocytes/macrophages, mast cells, neutrophils, and lymphocytes. Recombinant Galectin-3 is a globular 26.0 kDa protein containing 250 amino acid residues, but no disulfide bonds.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

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Bioinformatics

Gene Symbol LGALS3
Entrez