Recombinant Human Galectin-3 Protein

Product Discontinued

Recombinant Human Galectin-3 Protein Summary

• Description: Recombinant bioactive protein containing 250 amino acids for Human Galectin-3
  
  Source:E. coli
  Amino Acid Sequence:(P17931)
  
  This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab285623)
• Preparation Method: Determined to be >98% pure by SDS-PAGE
• Details of Functionality: Determined by its ability to chemoattract human blood monocytes. Chemotactic activity was observed at a concentration of 2.5 ug/ml with a peak response obtained at 250 ug/ml.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: LGALS3
• Purity: >98%, by SDS-PAGE
• Endotoxin Note: <0.1 ng/ug

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Application Notes: Unit Definition: One unit is defined as the amount of enzyme that hydrolyzes 1 nmol/min of Ac-IETD-PNA per min at 25oC in 50 mM Tris pH 8.0 containing 150 mM NaCl and 0.01% Triton X-100.
• Theoretical MW: 26 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: Sterile filtered and lyophilized from a concentrated solution containing 0.5X PBS (pH 7.2)
• Concentration: Lyoph
• Purity: >98%, by SDS-PAGE
• Reconstitution Instructions: Reconstitute in H2O to a concentration of 0.1 - 1.0 mg/ml. This solution can then be diluted into other aqueous buffers andstored at 4C for 1 week or -20C for long-term storage.

Alternate Names for Recombinant Human Galectin-3 Protein

• AGE-R3
• Carbohydrate-binding protein 35
• CBP 35,35 kDa lectin
• CBP35
• GAL3
• Gal-3
• galactin-3
• Galactose-specific lectin 3
• Galactoside-binding protein
• GALBPCBP35
• Galectin3
• Galectin-3
• GALIG
• IgE-binding protein
• L29
• L31
• L-31
• Laminin-binding protein
• Lectin L-29
• lectin, galactoside-binding, soluble, 3
• LGALS2
• LGALS3
• Mac-2 antigen
• Mac-2
• MAC2GAL3

Background

Lectins are carbohydrate binding proteins that interact with glycoprotein and glycolipids on the surface of animal cells. The Galectins are lectins that recognize and interact with Beta -galactoside moieties. Galactin-3 regulates a number of biological processes, including embryogenesis, inflammatory responses, cell progression and metastasis. Galectin-3 can function intraCellularly in controlling cell cycle and preventing T-cell apoptosis, and also extraCellularly, in activating various cells, including monocytes/macrophages, mast cells, neutrophils, and lymphocytes. Recombinant Galectin-3 is a globular 26.0 kDa protein containing 250 amino acid residues, but no disulfide bonds.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: LGALS3