Galectin-3 Antibody (M3/38 (M3/38)) [PE]

Product Discontinued

Galectin-3 Antibody (M3/38 (M3/38)) [PE] Summary

• Immunogen: The immunogen for this antibody was Galectin3 Mac2.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: LGALS3
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
• Application Notes: This eBioM3/38 (M3/38) antibody has been tested by flow cytometric analysis of mouse thioglycolate elicited peritoneal macrophages. This can be used at less than or equal to 0.06 ug per test. A test is defined as the amount (ug)/test of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Reactivity Notes

This antibody is reactive to Mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS [pH 7.2]
• Preservative: 0.09% Sodium Azide
• Purity: Protein A or G purified

Notes

Image Caption - Staining of C57BL/6 bone marrow cells with Anti-Mouse CD11b FITC and 0.25 ug of Rat IgG2a k Isotype Control PE (left) or 0.03 ug of Anti-Human/Mouse Galectin-3 PE (right). Total viable cells were used for analysis.

Alternate Names for Galectin-3 Antibody (M3/38 (M3/38)) [PE]

• AGE-R3
• Carbohydrate-binding protein 35
• CBP 35,35 kDa lectin
• CBP35
• GAL3
• Gal-3
• galactin-3
• Galactose-specific lectin 3
• Galactoside-binding protein
• GALBPCBP35
• Galectin3
• Galectin-3
• GALIG
• IgE-binding protein
• L29
• L31
• L-31
• Laminin-binding protein
• Lectin L-29
• lectin, galactoside-binding, soluble, 3
• LGALS2
• LGALS3
• Mac-2 antigen
• Mac-2
• MAC2GAL3

Background

The M3/38 antibody reacts with mouse Galectin-3 (Mac-2). Galectins are a family of animal lectins which appear to exhibit a variety of biological functions. Galectin 3 (aka LGALS3, galactose-specific soluble lectin 3, Mac-2, L-29) is one of the more extensively studied members of this family and is a 30 kDa beta-galactoside-binding protein. Due to a C-terminal carbohydrate binding site, Galectin 3 is capable of binding IgE and mammalian cell surfaces only when homodimerized or homo-oligomerized. Galectin 3 is normally distributed in epithelia of many organs and various inflammatory cells, including macrophages, as well as dendritic cells and Kupffer cells. The expression of this lectin is up-regulated during inflammation, cell proliferation, cell differentiation and through trans-activation by viral proteins. This monoclonal antibody M3/38 crossreacts to human Galectin-3 (LGALS3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LGALS3