Galanin R1/GALR1 Antibody

Western Blot: Galanin R1/GALR1 Antibody [NB100-57080] - (0.1ug/ml) staining of Rat Spinal Cord lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Rt, Hu, Mu, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Galanin R1/GALR1 Antibody Summary

• Description: Novus Biologicals Goat Galanin R1/GALR1 Antibody (NB100-57080) is a polyclonal antibody validated for use in WB and ELISA. Anti-Galanin R1/GALR1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-RSKPGKPRSTTN corresponding to internal region according to NP_001471.2.
• Epitope: RSKPGKPRSTTN
• Predicted Species: Human (100%), Mouse (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GALR1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 24-25 kDa band and 37 kDa band bands observed in lysates of rat spinal cord (calculated MW of 24.9 kDa band according to rat NP_037090.1 and calculated MW of 38.9 kDa band according to AAC52438.1 and sp Q62805).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Galanin R1/GALR1 Antibody

• GAL1
• Galanin R1
• galanin receptor 1
• galanin receptor type 1
• GalaninR1
• GALNR1
• GALNRGALNR1GAL1-R
• GALR1
• GALR-1

Background

The neuropeptide galanin elicits a range of biological effects by interaction with specific G-protein-coupled receptors. Galanin receptors are seven-transmembrane proteins shown to activate a variety of intracellular second-messenger pathways. GALR1 inhibits adenylyl cyclase via a G protein of the Gi/Go family. GALR1 is widely expressed in the brain and spinal cord, as well as in peripheral sites such as the small intestine and heart.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Galanin R1/GALR1 Antibody (NB100-57080)(1)

Publication using NB100-57080

• Henson BS, Neubig RR, Jang I et al. Galanin receptor 1 has anti-proliferative effects in oral squamous cell carcinoma. J Biol Chem 2005-06-17 [PMID: 15767248]

Bioinformatics

• Gene Symbol: GALR1
• Entrez:
  • 2587(Human)
  • 14427(Mouse)
  • 50577(Rat)
• Uniprot:
  • NP_001471.1(Human)