Recombinant Human GADD45G Protein

SDS-Page: GADD45G Protein [NBC1-18437] - GADD45G, 17.1 kDa (159 aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human GADD45G Protein Summary

• Description: An un-tagged recombinant protein corresponding to the amino acids 1-159 of Human GADD45G
  
  

  Source: E.coli

  Amino Acid Sequence: MTLEEVRGQD TVPESTARMQ GAGKALHELL LSAQRQGCLT AGVYESAKVL NVDPDNVTFC VLAAGEEDEG DIALQIHFTL IQAFCCENDI DIVRVGDVQR LAAIVGAGEE AGAPGDLHCI LISNPNEDAW KDPALEKLSL FCEESRSVND WVPSITLPE

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: GADD45G
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 17.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 7.5)
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human GADD45G Protein

• CR6DNA damage-inducible transcript 2 protein
• Cytokine-responsive protein CR6
• DDIT-2
• DDIT2GADD45-gamma
• growth arrest and DNA damage-inducible protein GADD45 gamma
• growth arrest and DNA-damage-inducible, gamma
• GRP1717 kD

Background

GADD45G is a member of GADD45 family (GADD45alpha, beta, and gamma) that are nuclear proteins to interact with various proteins implicated in stress responses and cell-cycle-related proteins. The function of GADD45 proteins is involved in growth-regulatory mechanisms and apoptosis. GADD45G protein responds to environmental stresses by mediating activation of p38/JNK pathway via MTK1/MEKK4 kinase and its transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. Recombinant GADD45G protein was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: GADD45G