Human GADD153/CHOP - Ready-To-Use ELISA Kit (Colorimetric)

ELISA: Human GADD153/CHOP - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31438] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Standard Curve Range: 0.312 - 20 ng/mL (example only; lot dependent)
• Sensitivity: 0.138 ng/mL (example only; lot dependent)

Human GADD153/CHOP - Ready-To-Use ELISA Kit (Colorimetric) Summary

• Description: The Ready-To-Use ELISA kit offers pre-diluted detection reagents and a shorter experimental time.
  Assay Length: 3 hours
• Standard Curve Range: 0.312 - 20 ng/mL (example only; lot dependent)
• Sensitivity: 0.138 ng/mL (example only; lot dependent)
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 12 (example only; lot dependent)
• Intra-Assay: %CV < 10 (example only; lot dependent)
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: DDIT3

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human GADD153/CHOP - Ready-To-Use ELISA Kit (Colorimetric)

• C/EBP-homologous protein 10
• C/EBP-homologous protein
• CCAAT/enhancer-binding protein homologous protein
• CEBP zeta
• CHOP
• CHOP10 CEBPZ
• CHOP10
• CHOP-10
• CHOPC/EBP zeta
• DDIT3
• DDIT-3
• DNA damage-inducible transcript 3 protein
• DNA-damage-inducible transcript 3
• GADD153
• Growth arrest and DNA damage-inducible protein GADD153

Background

GADD153 encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: DDIT3