GAD65/67 Antibody (L127/12) [FL490] - Chimeric

• Reactivity: Hu, Mu, Rt
• Applications: ICC/IF, IHC
• Clone: L127/12
• Clonality: Monoclonal
• Host: Chicken
• Conjugate: FL490
• Concentration: 0.5 mg/ml

GAD65/67 Antibody (L127/12) [FL490] - Chimeric Summary

• Description: FL490 Excitation: 491 nm, Emission: 515nm.
  Each order is a unique lot. For specialized requirements, please inquire for available options.
  
  This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone L127/12 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone L127/12 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in HEK293 cells and then affinity purified from the cell culture media and conjugated.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Fusion protein amino acids 1-594 (full-length) of human GAD65/67 (Uniprot#: Q99259) produced recombinantly in E. Coli
• Specificity: Cross-reacts with GAD25, GAD44 and GAD65/GAD2
• Isotype: IgY
• Clonality: Monoclonal
• Host: Chicken
• Gene: GAD1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry-Paraffin 1:100
• Theoretical MW: 70 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 1X PBS (pH 7.4)
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Affinity purified

Alternate Names for GAD65/67 Antibody (L127/12) [FL490] - Chimeric

• 67kD)
• EC 4.1.1
• FLJ45882
• glutamate decarboxylase 1 (brain, 67kDa)
• Glutamate decarboxylase 67 kDa isoform

Background

GAD65/67 encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GAD1