GAD65/67 Antibody (JM11-11)

Western Blot: GAD65/67 Antibody (JM11-11) [NBP2-66855] - Western blot analysis of GAD67 on SH-SY5Y cells lysates using anti-GAD67 antibody at 1/500 dilution.

Western Blot: GAD65/67 Antibody (JM11-11) [NBP2-66855] - Analysis of GAD67 on Hela cells lysates using anti-GAD67 antibody at 1/500 dilution.

Product Discontinued

GAD65/67 Antibody (JM11-11) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human GAD65/67 aa 60-99 / 594. (SwissProt: Q99259 Human; SwissProt: P48318 Mouse; SwissProt: P18088 Rat)
• Localization: Cytoplasm, Plasma Membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: GAD1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:50-1:100
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for GAD65/67 Antibody (JM11-11)

• 67kD)
• EC 4.1.1
• FLJ45882
• glutamate decarboxylase 1 (brain, 67kDa)
• Glutamate decarboxylase 67 kDa isoform

Background

GAD65/67 encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GAD1