Mouse GAD2/GAD65 ELISA Kit (Colorimetric)

ELISA: Mouse GAD2/GAD65 ELISA Kit (Colorimetric) [NBP2-67968] - Samples were spiked with high concentrations of Mouse GAD2/GAD65 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Mouse GAD2/GAD65 ELISA Kit (Colorimetric) [NBP2-67968] - Standard Curve Reference

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.63 - 40 ng/mL
• Sensitivity: 0.38 ng/mL

Mouse GAD2/GAD65 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Mouse GAD2 in samples. No significant cross-reactivity or interference between Mouse GAD2 and analogues was observed.
• Standard Curve Range: 0.63 - 40 ng/mL
• Sensitivity: 0.38 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 4.58%
• Intra-Assay: CV% < 4.54%
• Spike Recovery: 87-106%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: GAD2

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse GAD2/GAD65 ELISA Kit (Colorimetric)

• EC 4.1.1
• EC 4.1.1.15
• GAD2
• GAD65
• GAD-65
• GAD65MGC161605
• glutamate decarboxylase 2 (pancreatic islets and brain, 65kD)
• glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)
• glutamate decarboxylase 2
• Glutamate decarboxylase 65 kDa isoform
• Glutamate decarboxylase-2 (pancreas)
• MGC161607,65 kDa glutamic acid decarboxylase

Background

Antibodies to glutamic acid decarboxylase-65 (GAD65) are present in autoimmune disorders such as insulin-dependent (type 1) diabetes mellitus (IDDM), stiff man syndrome and polyendocrine autoimmune disease. Auto-antibodies to GAD65 are present in 60-70% of individuals with newly diagnosed IDDM and thus are important markers for disease activity. These auto-antibodies usually recognize conformation-dependent regions on GAD65 and rarely bind to the second isoform, GAD67. Auto-antibodies to GAD67 are found in only 15% of recent-onset IDDM patients and most of this binding can be blocked with GAD65, suggesting shared epitopes between the two isoforms of GAD.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: GAD2