GAD2/GAD65 Antibody

Product Discontinued

GAD2/GAD65 Antibody Summary

• Immunogen: A synthetic peptide derived from the C-terminus region of the human GAD65 protein.
• Localization: Cytoplasmic
• Marker: GABAergic Neuronal Marker
• Specificity: This reacts with a 65 kD protein, known as GAD (Glutamate Decarboxylase)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GAD2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
• Application Notes: IHC: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.

Reactivity Notes

Cross-reacts with Human and Rat. Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS with BSA
• Preservative: Sodium Azide
• Purity: Protein A purified

Alternate Names for GAD2/GAD65 Antibody

• EC 4.1.1
• EC 4.1.1.15
• GAD2
• GAD65
• GAD-65
• GAD65MGC161605
• glutamate decarboxylase 2 (pancreatic islets and brain, 65kD)
• glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)
• glutamate decarboxylase 2
• Glutamate decarboxylase 65 kDa isoform
• Glutamate decarboxylase-2 (pancreas)
• MGC161607,65 kDa glutamic acid decarboxylase

Background

Two forms of GAD exist in adults, GAD 65 and GAD 67, which are encoded by different genes. GAD 65 and GAD 67 are expressed in most GABA containing neurons. GAD 65 appears to be targeted to the membrane and nerve ending, where as GAD 67 is more widely distributed throughout the cell.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GAD2/GAD65 Antibody (NB600-1093)(1)

Publication using NB600-1093

• Zhang S, Hu S, Dong W et al. Prenatal dexamethasone exposure induces anxiety- and depressive-like behavior of male offspring rats through intrauterine programming of the activation of NRG1-ErbB4 signaling in hippocampal PV interneurons Cell biology and toxicology 2021-06-29 [PMID: 34189720]

Bioinformatics

• Gene Symbol: GAD2
• Entrez:
  • 2572(Human)
  • 24380(Rat)