GAD2/GAD65 Antibody

Western Blot: GAD2/GAD65 Antibody [H00002572-B01P] - Analysis of GAD2 expression in rat brain.

Western Blot: GAD2/GAD65 Antibody [H00002572-B01P] - Analysis of GAD2 expression in transfected 293T cell line by GAD2 polyclonal antibody. Lane 1: GAD2 transfected lysate(64.35 KDa). Lane 2: Non-transfected lysate.

Product Discontinued

GAD2/GAD65 Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: GAD2 (NP_000809.1, 1 a.a. - 585 a.a.) full-length human protein. MASPGSGFWSFGSEDGSGDSENPGTARAWCQVAQKFTGGIGNKLCALLYGDAEKPAESGGSQPPRAAARKAACACDQKPCSCSKVDVNYAFLHATDLLPACDGERPTLAFLQDVMNILLQYVVKSFDRSTKVIDFHYPNELLQEYNWELADQPQNLEEILMHCQTTLKYAIKTGHPRYFNQLSTGLDMVGLAADWLTSTANTNMFTYEIAPVFVLLEYVTLKKMREIIGWPGGSGDGIFSPGGAISNMYAMMIARFKMFPEVKEKGMAALPRLIAFTSEHSHFSLKKGAAALGIGTDSVILIKCDERGKMIPSDLERRILEAKQKGFVPFLVSATAGTTVYGAFDPLLAVADICKKYKIWMHVDAAWGGGLLMSRKHKWKLSGVERANSVTWNPHKMMGVPLQCSALLVREEGLMQNCNQMHASYLFQQDKHYDLSYDTGDKALQCGRHVDVFKLWLMWRAKGTTGFEAHVDKCLELAEYLYNIIKNREGYEMVFDGKPQHTNVCFWYIPPSLRTLEDNEERMSRLSKVAPVIKARMMEYGTTMVSYQPLGDKVNFFRMVISNPAATHQDIDFLIEEIERLGQDL
• Marker: GABAergic Neuronal Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: GAD2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: It has been used for WB and Functional.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein G purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for GAD2/GAD65 Antibody

• EC 4.1.1
• EC 4.1.1.15
• GAD2
• GAD65
• GAD-65
• GAD65MGC161605
• glutamate decarboxylase 2 (pancreatic islets and brain, 65kD)
• glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)
• glutamate decarboxylase 2
• Glutamate decarboxylase 65 kDa isoform
• Glutamate decarboxylase-2 (pancreas)
• MGC161607,65 kDa glutamic acid decarboxylase

Background

Antibodies to glutamic acid decarboxylase-65 (GAD65) are present in autoimmune disorders such as insulin-dependent (type 1) diabetes mellitus (IDDM), stiff man syndrome and polyendocrine autoimmune disease. Auto-antibodies to GAD65 are present in 60-70% of individuals with newly diagnosed IDDM and thus are important markers for disease activity. These auto-antibodies usually recognize conformation-dependent regions on GAD65 and rarely bind to the second isoform, GAD67. Auto-antibodies to GAD67 are found in only 15% of recent-onset IDDM patients and most of this binding can be blocked with GAD65, suggesting shared epitopes between the two isoforms of GAD.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GAD2
• Uniprot:
  • NP_000809.1(Human)